Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy

Michihiko Nakano, Z. Ding, H. Kasahara, Junya Suehiro

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

This paper reports a new method to determine the size of DNA amplified by polymerase chain reaction (PCR). After PCR, the amplified DNA was attached on dielectric microbeads, then, the DNA labeled microbeads were trapped on a microelectrode by dielectrophoresis (DEP). The size of the DNA was determined by measuring frequency dependent impedance of the trapped microbeads as an impedance spectroscopy. The size of various DNAs, 142, 204, 391, 796 bp, were successfully identified from the impedance spectrograms. This method determines DNA size in 20 min after PCR.

元の言語英語
ホスト出版物のタイトル2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
出版者Institute of Electrical and Electronics Engineers Inc.
ページ1530-1532
ページ数3
ISBN(電子版)9781479989553
DOI
出版物ステータス出版済み - 8 5 2015
イベント18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, 米国
継続期間: 6 21 20156 25 2015

出版物シリーズ

名前2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

その他

その他18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
米国
Anchorage
期間6/21/156/25/15

Fingerprint

Size determination
size determination
polymerase chain reaction
Polymerase chain reaction
beads
DNA
deoxyribonucleic acid
Spectroscopy
impedance
spectroscopy
spectrograms
Microelectrodes
Electrophoresis

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

これを引用

Nakano, M., Ding, Z., Kasahara, H., & Suehiro, J. (2015). Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. : 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 1530-1532). [7181228] (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181228

Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. / Nakano, Michihiko; Ding, Z.; Kasahara, H.; Suehiro, Junya.

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1530-1532 7181228 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

研究成果: 著書/レポートタイプへの貢献会議での発言

Nakano, M, Ding, Z, Kasahara, H & Suehiro, J 2015, Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. : 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181228, 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Institute of Electrical and Electronics Engineers Inc., pp. 1530-1532, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, 米国, 6/21/15. https://doi.org/10.1109/TRANSDUCERS.2015.7181228
Nakano M, Ding Z, Kasahara H, Suehiro J. Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. : 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1530-1532. 7181228. (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). https://doi.org/10.1109/TRANSDUCERS.2015.7181228
Nakano, Michihiko ; Ding, Z. ; Kasahara, H. ; Suehiro, Junya. / Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1530-1532 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).
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