Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Kiichiro Teruya, Takahiro Yano, Sanetaka Shirahata, Junko Watanabe, Kazuhiro Osada, Hirofumi Tachibana, Hiroki Murakami, Hideya Ohashi, Eun Ho Kim

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.

本文言語英語
ページ(範囲)341-344
ページ数4
ジャーナルBioscience, biotechnology, and biochemistry
59
2
DOI
出版ステータス出版済み - 1995

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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