Rat monoclonal antibody specific for MyoD

Akihito Harada, Yasuyuki Ohkawa, Shinpei Ao, Jun Odawara, Seiji Okada, Masayuki Azuma, Yuko Nishiyama, Mako Nakamura, Taro Tachibana

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.

元の言語英語
ページ(範囲)255-258
ページ数4
ジャーナルHybridoma
29
発行部数3
DOI
出版物ステータス出版済み - 6 1 2010

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Skeletal Muscle
Monoclonal Antibodies
Helix-Loop-Helix Motifs
Myogenic Regulatory Factors
Myoblasts
Regulator Genes
Transcription Factors
Lymph Nodes
Staining and Labeling
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

これを引用

Harada, A., Ohkawa, Y., Ao, S., Odawara, J., Okada, S., Azuma, M., ... Tachibana, T. (2010). Rat monoclonal antibody specific for MyoD. Hybridoma, 29(3), 255-258. https://doi.org/10.1089/hyb.2009.0117

Rat monoclonal antibody specific for MyoD. / Harada, Akihito; Ohkawa, Yasuyuki; Ao, Shinpei; Odawara, Jun; Okada, Seiji; Azuma, Masayuki; Nishiyama, Yuko; Nakamura, Mako; Tachibana, Taro.

:: Hybridoma, 巻 29, 番号 3, 01.06.2010, p. 255-258.

研究成果: ジャーナルへの寄稿記事

Harada, A, Ohkawa, Y, Ao, S, Odawara, J, Okada, S, Azuma, M, Nishiyama, Y, Nakamura, M & Tachibana, T 2010, 'Rat monoclonal antibody specific for MyoD', Hybridoma, 巻. 29, 番号 3, pp. 255-258. https://doi.org/10.1089/hyb.2009.0117
Harada, Akihito ; Ohkawa, Yasuyuki ; Ao, Shinpei ; Odawara, Jun ; Okada, Seiji ; Azuma, Masayuki ; Nishiyama, Yuko ; Nakamura, Mako ; Tachibana, Taro. / Rat monoclonal antibody specific for MyoD. :: Hybridoma. 2010 ; 巻 29, 番号 3. pp. 255-258.
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AU - Odawara, Jun

AU - Okada, Seiji

AU - Azuma, Masayuki

AU - Nishiyama, Yuko

AU - Nakamura, Mako

AU - Tachibana, Taro

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