Rational Design Principles of Attenuated Cationic Lytic Peptides for Intracellular Delivery of Biomacromolecules

Naoki Tamemoto, Naoki Tamemoto, Misao Akishiba, Kentarou Sakamoto, Kenichi Kawano, Hiroshi Noguchi, Shiroh Futaki

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)

抄録

Intracellular delivery of bioactive macromolecules via endocytic pathways has utility in biotechnological and medicinal applications. Various endosomolytic peptides bearing glutamic acid (Glu) residues have been developed with the aim to achieve selective lysis of endosomal membranes without damaging cell membranes (plasma membranes) to release endosome-entrapped macromolecules and obtain their bioactivity. Glu residues on peptides are negatively charged in the extracellular medium, and substitution of this residue onto membrane-lytic peptides prevents its peptide-membrane interaction and its lytic activity. On the other hand, within endosomes, which have a reduced pH of ∼5, Glu is protonated, resulting in the reduction of the hydrophilicity of the peptide, unmasking its lytic activity. Despite this, a limited number of studies have elucidated the optimum positions for Glu substitution. This report investigated the positioning of Glu and the endosomolytic activities of cationic lytic peptides, ponericin-W3, and melittin. By cell-based assays, biophysical analyses, and molecular dynamics simulations, we found that analogues with Glu positioned on the borders between the hydrophobic and hydrophilic faces of the helical structures showed better performance than placing Glu within said faces.

本文言語英語
ページ(範囲)2175-2185
ページ数11
ジャーナルMolecular pharmaceutics
17
6
DOI
出版ステータス出版済み - 6月 1 2020
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分子医療
  • 薬科学
  • 創薬

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