Real-time fluorescence monitoring of GSK3β-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor

Takashi Sakamoto, Masa aki Inoue, Akio Ojida, Itaru Hamachi

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

We developed a new fluorescence assay system for GSK3β-catalyzed kinase reaction using the BODIPY-based fluorescent chemosensor. This system exploits the selective sensing property of the chemosensor for a (i, i+4) bis-phosphorylated peptide, which allows us to conveniently detect the phosphorylation reaction with a fluorescence increase in a real-time fashion.

元の言語英語
ページ(範囲)4175-4177
ページ数3
ジャーナルBioorganic and Medicinal Chemistry Letters
19
発行部数15
DOI
出版物ステータス出版済み - 8 1 2009
外部発表Yes

Fingerprint

Fluorescence
Phosphorylation
Monitoring
Assays
Phosphotransferases
Peptides
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

Real-time fluorescence monitoring of GSK3β-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor. / Sakamoto, Takashi; Inoue, Masa aki; Ojida, Akio; Hamachi, Itaru.

:: Bioorganic and Medicinal Chemistry Letters, 巻 19, 番号 15, 01.08.2009, p. 4175-4177.

研究成果: ジャーナルへの寄稿記事

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