Recent progress toward RNA manipulation with engineered pentatricopeptide repeat proteins

Takayoshi Imai, Yusuke Yagi, Takahiro Nakamura

研究成果: 書籍/レポート タイプへの寄稿

1 被引用数 (Scopus)

抄録

Pentatricopeptide repeat (PPR) proteins are RNA-binding proteins that are widely distributed in plants. They contain 2 to 30 repeating units of ~35-amino acid PPR motifs. They are known to play important roles in RNA processing, RNA editing, and translational regulation. Recent studies on the RNA recognition mode of PPR proteins revealed that one PPR motif interacts with one nucleotide. In addition, it was revealed that amino acids at three specific positions in a single motif serve to specify its binding base. Thus, mutation of these amino acids can cause a modification of the binding specificity of PPR motifs. Indeed, the engineered PPR motifs fused with various effector domains are shown to bind to and manipulate RNAs in a controlled manner. In this review, we summarize the recent progress in structural studies on PPR motifs. We focus on their RNA recognition mode and discuss the potentials of PPR as novel, versatile tools for RNA manipulation.

本文言語英語
ホスト出版物のタイトルApplied RNA Bioscience
出版社Springer Singapore
ページ151-160
ページ数10
ISBN(電子版)9789811083723
ISBN(印刷版)9789811083716
DOI
出版ステータス出版済み - 4月 10 2018

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 医学(全般)

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