Receptor selectivity of natriuretic peptide family, atrial natriuretic peptide, brain natriuretic peptide, and c-type natriuretic peptide

Shin Ichi Suga, Kazuwa Nakao, Kiminori Hosoda, Masahi Mukoyama, Yoshihiro Ogawa, Gotaro Shirakami, Hiroshi Arai, Yoshihiko Saito, Yoshikazu Kambayashi, Ken Inouye, Hiroo Imura

研究成果: ジャーナルへの寄稿学術誌査読

663 被引用数 (Scopus)

抄録

To elucidate the ligand-receptor relationship of the natriuretic peptide system, which comprises at least three endogenous ligands, atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP), and three receptors, the ANP-A receptor or guanylate cyclase-A (GC-A), the ANP -B receptor or guanylate cyclase-B (GC-B), and the clearance receptor (C-receptor), we characterized the receptor preparations from human, bovine, and rat tissues and cultured cells with the aid of the binding assay, Northern blot technique, and the cGMP production method. Using these receptor preparations, we examined the binding affinities of ANP, BNP, and CNP for the C-receptor and their potencies for cGMP production via the ANP-A receptor (GC-A) and the ANP-B receptor (GC-B). These analyses revealed the presence of a marked species difference in the receptor selectivity of the natriuretic peptide family, especially among BNPs. Therefore, we investigated the receptor selectivity of the natriuretic peptide family using the homologous assay system with endogenous ligands and receptors of the same species. The rank order of binding affinity for the C-receptor was ANP > CNP > BNP in both humans and rats. The rank order of potency for cGMP production via the ANP-A receptor (GC-A) was ANP greater than or equal to BNP ˃˃ CNP, but that via the ANP-B receptor (GC-B) was CNP ˃ ANP greater than or equal to BNP. These findings on the receptor selectivity of the natriuretic peptide family provide a new insight into the understanding of the physiological and clinical implications of the natriuretic peptide system.

本文言語英語
ページ(範囲)229-239
ページ数11
ジャーナルEndocrinology
130
1
DOI
出版ステータス出版済み - 1月 1992
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 内分泌学

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