Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks

Yuri Tsuji, Kenji Watanabe, Kimi Araki, Masanori Shinohara, Yuriko Yamagata, Toshiki Tsurimoto, Fumio Hanaoka, Ken ichi Yamamura, Masaru Yamaizumi, Satoshi Tateishi

研究成果: ジャーナルへの寄稿記事

41 引用 (Scopus)

抄録

Post-replication DNA repair facilitates the resumption of DNA synthesis upon replication fork stalling at DNA damage sites. Despite the importance of RAD18 and polymerase η (Polη) for post-replication repair (PRR), the molecular mechanisms by which these factors are recruited to stalled replication forks are not well understood. We present evidence that human RAD18 complexed with RAD6B protein preferentially binds to forked and single-stranded DNA (ssDNA) structures, which are known to be localized at stalled replication forks. The SAP domain of RAD18 (residues 248-282) is crucial for binding of RAD18 complexed with RAD6B to DNA substrates. RAD18 mutated in the SAP domain fails to accumulate at DNA damage sites in vivo and does not guide DNA Polη to stalled replication forks. The SAP domain is also required for the efficient mono-ubiquitination of PCNA. The SAP domain mutant fails to suppress the ultraviolet (UV)-sensitivity of Rad18 -knockout cells. These results suggest that RAD18 complexed with RAD6B is recruited to stalled replication forks via interactions with forked DNA or long ssDNA structures, a process that is required for initiating PRR.

元の言語英語
ページ(範囲)343-354
ページ数12
ジャーナルGenes to Cells
13
発行部数4
DOI
出版物ステータス出版済み - 4 1 2008

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Single-Stranded DNA
DNA Damage
DNA
Proteins
Ubiquitination
Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
DNA Repair

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

これを引用

Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. / Tsuji, Yuri; Watanabe, Kenji; Araki, Kimi; Shinohara, Masanori; Yamagata, Yuriko; Tsurimoto, Toshiki; Hanaoka, Fumio; Yamamura, Ken ichi; Yamaizumi, Masaru; Tateishi, Satoshi.

:: Genes to Cells, 巻 13, 番号 4, 01.04.2008, p. 343-354.

研究成果: ジャーナルへの寄稿記事

Tsuji, Y, Watanabe, K, Araki, K, Shinohara, M, Yamagata, Y, Tsurimoto, T, Hanaoka, F, Yamamura, KI, Yamaizumi, M & Tateishi, S 2008, 'Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks', Genes to Cells, 巻. 13, 番号 4, pp. 343-354. https://doi.org/10.1111/j.1365-2443.2008.01176.x
Tsuji, Yuri ; Watanabe, Kenji ; Araki, Kimi ; Shinohara, Masanori ; Yamagata, Yuriko ; Tsurimoto, Toshiki ; Hanaoka, Fumio ; Yamamura, Ken ichi ; Yamaizumi, Masaru ; Tateishi, Satoshi. / Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. :: Genes to Cells. 2008 ; 巻 13, 番号 4. pp. 343-354.
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