Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM+ B cells

Takuya Yamaguchi, Shunsuke Miyata, Fumihiko Katakura, Takahiro Nagasawa, Yasuhiro Shibasaki, Takeshi Yabu, Uwe Fischer, Chihaya Nakayasu, Teruyuki Nakanishi, Tadaaki Moritomo

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp (Cyprinus carpio) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM+ B cells, because after four days of stimulation the IgM+ fraction of carp kidney and spleen leukocytes had formed many cell colonies, whereas such colonies were not found in the absence of rcIL-4/13B stimulation. After nine days of incubation with rcIL-4/13B these cells had proliferated to more than 3-to-7-fold higher numbers when compared to untreated cells. The proliferating cells contained a majority of IgM+ cells but also other cells, as indicated by FACS and RT-PCR analyses. The important conclusion is that in fish not only IL-4/13A has B cell stimulating properties, as a previous publication has shown, but also IL-4/13B.

元の言語英語
ページ(範囲)225-229
ページ数5
ジャーナルFish and Shellfish Immunology
49
DOI
出版物ステータス出版済み - 2 1 2016

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interleukin-4
carp
Interleukin-4
B-lymphocytes
Immunoglobulin M
Cells
protein
teleost
incubation
cells
fold
Cyprinus carpio
gene
fish
Interleukin-13
Recombinant Proteins
Fish
recombinant proteins
Genes
leukocytes

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

これを引用

Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM+ B cells. / Yamaguchi, Takuya; Miyata, Shunsuke; Katakura, Fumihiko; Nagasawa, Takahiro; Shibasaki, Yasuhiro; Yabu, Takeshi; Fischer, Uwe; Nakayasu, Chihaya; Nakanishi, Teruyuki; Moritomo, Tadaaki.

:: Fish and Shellfish Immunology, 巻 49, 01.02.2016, p. 225-229.

研究成果: ジャーナルへの寄稿記事

Yamaguchi, T, Miyata, S, Katakura, F, Nagasawa, T, Shibasaki, Y, Yabu, T, Fischer, U, Nakayasu, C, Nakanishi, T & Moritomo, T 2016, 'Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM+ B cells', Fish and Shellfish Immunology, 巻. 49, pp. 225-229. https://doi.org/10.1016/j.fsi.2015.12.043
Yamaguchi, Takuya ; Miyata, Shunsuke ; Katakura, Fumihiko ; Nagasawa, Takahiro ; Shibasaki, Yasuhiro ; Yabu, Takeshi ; Fischer, Uwe ; Nakayasu, Chihaya ; Nakanishi, Teruyuki ; Moritomo, Tadaaki. / Recombinant carp IL-4/13B stimulates in vitro proliferation of carp IgM+ B cells. :: Fish and Shellfish Immunology. 2016 ; 巻 49. pp. 225-229.
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AU - Shibasaki, Yasuhiro

AU - Yabu, Takeshi

AU - Fischer, Uwe

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AU - Nakanishi, Teruyuki

AU - Moritomo, Tadaaki

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