Recombinant Sendai virus vectors for activated T lymphocytes

S. Okano, Y. Yonemitsu, S. Nagata, S. Sata, M. Onimaru, K. Nakagawa, Y. Tomita, K. Kishihara, S. Hashimoto, Y. Nakashima, K. Sugimachi, M. Hasegawa, K. Sueishi

研究成果: ジャーナルへの寄稿学術誌査読

20 被引用数 (Scopus)

抄録

T-lymphocyte-directed gene therapy has potential as a treatment of subjects with immunological disorders. One current limitation of this therapeutic strategy is low gene transfer efficiency, even when complex procedures are used. We report herein that a recombinant Sendai virus vector (SeV) was able to overcome this issue. Using jellyfish enhanced green fluorescent protein gene (EGFP), we found that SeV was able to transduce and express a foreign gene specifically and efficiently in activated murine and human T cells, but not in naive T cells, without centrifugation or reagents including polybrene and protamine sulfate; the present findings were in clear contrast to those demonstrated with the use of retroviruses. The transduction was selective in antigen-activated T cells, while antigen-irrelevant T cells were not transduced, even under bystander activation from specific T-cell responses by antigens ex vivo. Receptor saturation studies suggested a possible mechanism of activated T-cell-specific gene transfer, ie, SeV might attach to naive T cells but might be unable to enter their cytoplasm. We therefore propose that the SeV vector system may prove to be a potentially important alternative in the area of T-cell-directed gene therapy used in the clinical setting.

本文言語英語
ページ(範囲)1381-1391
ページ数11
ジャーナルGene Therapy
10
16
DOI
出版ステータス出版済み - 8月 2003

!!!All Science Journal Classification (ASJC) codes

  • 分子医療
  • 分子生物学
  • 遺伝学

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