Reconstitution activity of hypoxic cultured human cord blood CD34-positive cells in NOG mice

Haruko Shima, Keiyo Takubo, Hiroko Iwasaki, Hiroki Yoshihara, Yumiko Gomei, Kentaro Hosokawa, Fumio Arai, Takao Takahashi, Toshio Suda

研究成果: Contribution to journalArticle査読

37 被引用数 (Scopus)

抄録

Hematopoietic stem cells (HSCs) reside in hypoxic areas of the bone marrow. However, the role of hypoxia in the maintenance of HSCs has not been fully characterized. We performed xenotransplantation of human cord blood cells cultured in hypoxic or normoxic conditions into adult NOD/SCID/IL-2Rγnull (NOG) mice. Hypoxic culture (1% O2) for 6 days efficiently supported the maintenance of HSCs, although cell proliferation was suppressed compared to the normoxic culture. In contrast, hypoxia did not affect in vitro colony-forming ability. Upregulation of a cell cycle inhibitor, p21, was observed in hypoxic culture. Immunohistochemical analysis of recipient bone marrow revealed that engrafted CD34+CD38- cord blood HSCs were hypoxic. Taken together, these results demonstrate the significance of hypoxia in the maintenance of quiescent human cord blood HSCs.

本文言語英語
ページ(範囲)467-472
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
378
3
DOI
出版ステータス出版済み - 1 16 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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