Reconstitution of biosynthetic machinery of fungal polyketides: Unexpected oxidations of biosynthetic intermediates by expression host

Ryuya Fujii, Takahiro Ugai, Hirofumi Ichinose, Mayumi Hatakeyama, Takuto Kosaki, Katsuya Gomi, Isao Fujii, Atsushi Minami, Hideaki Oikawa

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Reconstitution of whole biosynthetic genes in Aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. Heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. In the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic genes. In this paper, we reported structure determination of by-products and described a simple solution to avoid the undesired reaction by introducing the downstream gene in the heterologous production of solanapyrone C.

元の言語英語
ページ(範囲)426-431
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
80
発行部数3
DOI
出版物ステータス出版済み - 1 1 2016

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Polyketides
Machinery
Genes
Oxidation
Byproducts
Cytochalasins
Aspergillus oryzae
Aspergillus
Biosynthesis
Alkenes
Enzymes
Metabolites
Biological Products

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Reconstitution of biosynthetic machinery of fungal polyketides : Unexpected oxidations of biosynthetic intermediates by expression host. / Fujii, Ryuya; Ugai, Takahiro; Ichinose, Hirofumi; Hatakeyama, Mayumi; Kosaki, Takuto; Gomi, Katsuya; Fujii, Isao; Minami, Atsushi; Oikawa, Hideaki.

:: Bioscience, Biotechnology and Biochemistry, 巻 80, 番号 3, 01.01.2016, p. 426-431.

研究成果: ジャーナルへの寄稿記事

Fujii, Ryuya ; Ugai, Takahiro ; Ichinose, Hirofumi ; Hatakeyama, Mayumi ; Kosaki, Takuto ; Gomi, Katsuya ; Fujii, Isao ; Minami, Atsushi ; Oikawa, Hideaki. / Reconstitution of biosynthetic machinery of fungal polyketides : Unexpected oxidations of biosynthetic intermediates by expression host. :: Bioscience, Biotechnology and Biochemistry. 2016 ; 巻 80, 番号 3. pp. 426-431.
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