Reduced expression of the dysbindin -like gene in the Bombyx mori ov mutant exhibiting mottled translucency of the larval skin

Lingyan Wang, Takashi Kiuchi, Tsuguru Fujii, Takaaki Daimon, Muwang Li, Yutaka Banno, Susumu Katsuma, Toru Shimada

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

The ov (mottled translucent of Var) mutant, an oily mutant of Bombyx mori, exhibits mottled translucent skin with a varying degree of transparency among individuals. By linkage analysis of 2112 backcross individuals using polymorphic DNA markers, we successfully mapped a 179-kb region of chromosome 20 responsible for the ov phenotype. This region contains nine predicted genes. We compared the mRNA expression of these nine genes between the wild type and mutants and found that the expression of one of them, Bmdysb, was strikingly decreased in the epidermis of ov as well as its allelomorph, ovp. Moreover, its expression level was well correlated with the degree of transparency among individuals. Bmdysb was homologous to DTNBP1 encoding human dysbindin, a subunit of the biogenesis of lysosome-related organelles complex-1. Our results suggest that the translucent skin may be due to repression of Bmdysb in the ov mutants and that Bmdysb plays an important role in the formation and accumulation of urate granules in the silkworm epidermis.

元の言語英語
ページ(範囲)101-108
ページ数8
ジャーナルGenome
56
発行部数2
DOI
出版物ステータス出版済み - 2 1 2013

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Bombyx
Epidermis
Chromosomes, Human, Pair 20
Skin
Lysosomes
Uric Acid
Genetic Markers
Organelles
Genes
Alleles
Phenotype
Gene Expression
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics

これを引用

Reduced expression of the dysbindin -like gene in the Bombyx mori ov mutant exhibiting mottled translucency of the larval skin. / Wang, Lingyan; Kiuchi, Takashi; Fujii, Tsuguru; Daimon, Takaaki; Li, Muwang; Banno, Yutaka; Katsuma, Susumu; Shimada, Toru.

:: Genome, 巻 56, 番号 2, 01.02.2013, p. 101-108.

研究成果: ジャーナルへの寄稿記事

Wang, Lingyan ; Kiuchi, Takashi ; Fujii, Tsuguru ; Daimon, Takaaki ; Li, Muwang ; Banno, Yutaka ; Katsuma, Susumu ; Shimada, Toru. / Reduced expression of the dysbindin -like gene in the Bombyx mori ov mutant exhibiting mottled translucency of the larval skin. :: Genome. 2013 ; 巻 56, 番号 2. pp. 101-108.
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abstract = "The ov (mottled translucent of Var) mutant, an oily mutant of Bombyx mori, exhibits mottled translucent skin with a varying degree of transparency among individuals. By linkage analysis of 2112 backcross individuals using polymorphic DNA markers, we successfully mapped a 179-kb region of chromosome 20 responsible for the ov phenotype. This region contains nine predicted genes. We compared the mRNA expression of these nine genes between the wild type and mutants and found that the expression of one of them, Bmdysb, was strikingly decreased in the epidermis of ov as well as its allelomorph, ovp. Moreover, its expression level was well correlated with the degree of transparency among individuals. Bmdysb was homologous to DTNBP1 encoding human dysbindin, a subunit of the biogenesis of lysosome-related organelles complex-1. Our results suggest that the translucent skin may be due to repression of Bmdysb in the ov mutants and that Bmdysb plays an important role in the formation and accumulation of urate granules in the silkworm epidermis.",
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AU - Kiuchi, Takashi

AU - Fujii, Tsuguru

AU - Daimon, Takaaki

AU - Li, Muwang

AU - Banno, Yutaka

AU - Katsuma, Susumu

AU - Shimada, Toru

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