Reduced immunogenicity of monomethoxypolyethylene glycol-modified lysozyme for activation of T cells

Takanori So, Hiro O. Ito, Toshitaka Koga, Tadashi Ueda, Taiji Imoto

研究成果: Contribution to journalArticle査読

23 被引用数 (Scopus)

抄録

Chemical modification of proteins with monomethoxypolyethylene glycol (mPEG) will reduce the immunogenicity of proteins. In the present study, we evaluated the effect of mPEG modification on the capacity of hen egg-white lysozyme (HEL) to stimulate T cells. Lymph node cells (LNCs) from mice immunized with HEL or with mPEG-HEL conjugate were cultured with these antigens, then we measured the proliferation and IL-2 production. mPEG-modification lowered the T cell-activating capacity of HEL, both in vitro and in vivo. Neither toxicity, nor antigen non-specific immunosuppressive capacity was observed with mPEG-HEL and unconjugated mPEG. Suppressor cells were unlikely to be generated in the mPEG-HEL-primed LNCs. We next examined the behavior of mPEG-HEL during antigen processing. The capacity of HEL and mPEG-HEL to be incorporated by live cells was much the same. However, the susceptibility to various proteases, including endosomal/lysosomal enzymes, was significantly decreased by mPEG modification. The increased resistance of mPEG-HEL to proteolytic degradation implied that the conjugate was poorly presented to T cells. This may be an important factor related to the low immunogenicity of mPEG modified proteins.

本文言語英語
ページ(範囲)91-97
ページ数7
ジャーナルImmunology Letters
49
1-2
DOI
出版ステータス出版済み - 1 1 1996

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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