Reduced positive feedback regulation between myosin crossbridge and cardiac troponin C in fast skeletal myofibrils

Sachio Morimoto, Iwao Ohtsuki

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

Several studies have shown that substitution of cardiac troponin C into fast skeletal muscle causes a marked reduction in cooperativity of Ca2+-activation of both myofibrillar ATPase and tension development. To clarify the underlying mechanisms, in the present study, Ca2+ binding to cardiac troponin C inserted into fast skeletal myofibrils was measured. Two classes of binding sites with different affinities (classes 1 and 2) were clearly identified, which were equivalent stoichiometrically to the two high-affinity sites (sites III and IV) and a single low-affinity site (site II) of troponin C, respectively. Ca2+ binding to class-2 sites and Ca2+-activation of myofibrillar ATPase occurred in roughly the same Ca2+ concentration range, indicating that site II is responsible for Ca2+-regulation. Myosin crossbridge interactions with actin, both in the presence and absence of ATP, enhanced the Ca2+ binding affinity of only class-2 sites. These effects of myosin crossbridges, however, were much smaller than the effects on the Ca2+ binding to the low-affinity sites of fast skeletal troponin C, which are responsible for regulating fast skeletal myofibrillar ATPase. These findings provide strong evidence that the reduction in the cooperative response to Ca2+ upon substituting cardiac troponin C into fast skeletal myofibrils is due to a decrease in the positive feedback interaction between myosin crossbridge attachment and Ca2+ binding to the regulatory site of troponin C.

本文言語英語
ページ(範囲)737-742
ページ数6
ジャーナルJournal of biochemistry
119
4
出版ステータス出版済み - 4 1 1996

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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