Regional distribution and postnatal changes of D-amino acids in rat brain

Kenji Hamase, Hiroshi Homma, Yuki Takigawa, Takeshi Fukushima, Tomofumi Santa, Kazuhiro Imai

研究成果: Contribution to journalArticle査読

155 被引用数 (Scopus)

抄録

Regional distribution of D-amino acids in rat brain was studied by the modified highly sensitive analytical method which was previously developed. The method includes fluorogenic derivatization of each amino acid, isolation of each amino acid by reverse-phase HPLC, followed by enantiomeric separation with Pirkle-type chiral stationary phases. D-Amino acid contents were determined in the cerebrum, cerebellum, hippocampus, medulla oblongata, pituitary gland and pineal gland. D-Aspartic acid was observed in the pineal gland (3524 ± 263 nmol/g, data are for male rats of 6 weeks of age) and the pituitary gland (80.5 ± 9.0 nmol/g). D-Serine was found in various regions of the brain except for the cerebellum and medulla oblongata. D-Alanine was observed exclusively in the pituitary gland (25.9 ± 4.4 nmol/g), whereas D-leucine was found in the pineal gland (3.4 ± 0.4 nmol/g) and the hippocampus (1.6 ± 0.07 nmol/g). No other D-amino acids were detected in the brain. The contents of D-aspartic acid in the pituitary gland and D-serine in the pineal gland were higher in female rats. In contrast the contents of D-alanine in the pituitary gland and D-leucine in the pineal gland and the hippocampus were higher in males. Postnatal changes of D-aspartic acid and D-leucine in the pineal gland and D-alanine in the pituitary gland were also investigated. The results described in this paper suggested that distinct regulatory mechanisms exist for individual D-amino acids in the corresponding region of rat brain.

本文言語英語
ページ(範囲)214-222
ページ数9
ジャーナルBiochimica et Biophysica Acta - General Subjects
1334
2-3
DOI
出版ステータス出版済み - 3 15 1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学

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