Regression of intestinal adenomas by vaccination with heat shock protein 105-pulsed bone marrow-derived dendritic cells in ApcMin/+ mice

Kazunori Yokomine, Tetsuya Nakatsura, Satoru Senju, Naomi Nakagata, Motozumi Minohara, Jun-Ichi Kira, Yutaka Motomura, Tatsuko Kubo, Yutaka Sasaki, Yasuharu Nishimura

研究成果: ジャーナルへの寄稿記事

12 引用 (Scopus)

抄録

Heat shock protein (HSP) 105 is overexpressed in various cancers, but is expressed at low levels in many normal tissues, except for the testis. A vaccination with HSP105-pulsed bone marrow-derived dendritic cells (BM-DC) induced antitumor immunity without causing an autoimmune reaction in a mouse model. Because ApcMin/+ mice develop multiple adenomas throughout the intestinal tract by 4 months of age, the mice provide a clinically relevant model of human intestinal tumor. In the present study, we investigated the efficacy of the HSP105-pulsed BM-DC vaccine on tumor regression in the ApcMin/+ mouse. Western blot and immunohistochemical analyses revealed that the tumors of the ApcMin/+ mice endogenously overexpressed HSP105. Immunization of the ApcMin/+ mice with a HSP105-pulsed BM-DC vaccine at 6, 8, and 10 weeks of age significantly reduced the number of small-intestinal polyps accompanied by infiltration of both CD4+ and CD8+ T cells in the tumors. Cell depletion experiments proved that both CD4+ and CD8+ T cells play a critical role in the activation of antitumor immunity induced by these vaccinations. These findings indicate that the HSP105-pulsed BM-DC vaccine can provide potent immunotherapy for tumors that appear spontaneously as a result of the inactivation of a tumor suppressor gene, such as in the ApcMin/+ mouse model.

元の言語英語
ページ(範囲)1930-1935
ページ数6
ジャーナルCancer Science
98
発行部数12
DOI
出版物ステータス出版済み - 12 1 2007

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Heat-Shock Proteins
Adenoma
Dendritic Cells
Vaccination
Bone Marrow
Neoplasms
Immunity
Intestinal Polyps
Vaccines
T-Lymphocytes
Cancer Vaccines
Tumor Suppressor Genes
Immunotherapy
Testis
Immunization
Western Blotting

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

これを引用

Regression of intestinal adenomas by vaccination with heat shock protein 105-pulsed bone marrow-derived dendritic cells in ApcMin/+ mice. / Yokomine, Kazunori; Nakatsura, Tetsuya; Senju, Satoru; Nakagata, Naomi; Minohara, Motozumi; Kira, Jun-Ichi; Motomura, Yutaka; Kubo, Tatsuko; Sasaki, Yutaka; Nishimura, Yasuharu.

:: Cancer Science, 巻 98, 番号 12, 01.12.2007, p. 1930-1935.

研究成果: ジャーナルへの寄稿記事

Yokomine, K, Nakatsura, T, Senju, S, Nakagata, N, Minohara, M, Kira, J-I, Motomura, Y, Kubo, T, Sasaki, Y & Nishimura, Y 2007, 'Regression of intestinal adenomas by vaccination with heat shock protein 105-pulsed bone marrow-derived dendritic cells in ApcMin/+ mice', Cancer Science, 巻. 98, 番号 12, pp. 1930-1935. https://doi.org/10.1111/j.1349-7006.2007.00612.x
Yokomine, Kazunori ; Nakatsura, Tetsuya ; Senju, Satoru ; Nakagata, Naomi ; Minohara, Motozumi ; Kira, Jun-Ichi ; Motomura, Yutaka ; Kubo, Tatsuko ; Sasaki, Yutaka ; Nishimura, Yasuharu. / Regression of intestinal adenomas by vaccination with heat shock protein 105-pulsed bone marrow-derived dendritic cells in ApcMin/+ mice. :: Cancer Science. 2007 ; 巻 98, 番号 12. pp. 1930-1935.
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