Regulated patterns of bacterial movements based on their secreted cellulose nanofibers interacting interfacially with ordered chitin templates

Tetsuo Kondo, Wakako Kasai, Masanobu Nojiri, Yukako Hishikawa, Eiji Togawa, Dwight Romanovicz, R. Malcolm Brown

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

Gluconacetobacter xylinus, a gram-negative bacterium that synthesizes and extrudes a cellulose nanofiber in SH media moves in random manners, resulting in 3D-network structure of the secreted nanofibers termed a pellicle. In this study, the bacterial movement was successfully regulated to be in a waving manner when cultured on ordered templates made of chitin. Interestingly, by addition of more cellulose into the chitin ordered templates, the waving pattern was getting close to a linear or straight manner. Real time video analysis and other visualization techniques clarified that the regulation of the moving manners was due to the interfacial interaction between the secreted nanofibers and the template surfaces. Furthermore, the changing of the pattern due to the cellulose content in the ordered templates appeared to depend on the magnitude of the interaction between the template and nanofibers. This regulated autonomous deposition of the fibers will build patterned 3D-structure with unique properties on the surface of the templates, leading to a novel type of nanotechnology using biological systems with biomolecular nano-templates to design 3D-structures.

本文言語英語
ページ(範囲)113-120
ページ数8
ジャーナルJournal of Bioscience and Bioengineering
114
1
DOI
出版ステータス出版済み - 7 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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