TY - JOUR
T1 - Regulation of body size and behavioral state of C. elegans by sensory perception and the egl-4 cGMP-dependent protein kinase
AU - Fujiwara, Manabi
AU - Sengupta, Piali
AU - McIntire, Steven L.
N1 - Funding Information:
We are grateful to Hoan Phan, Nasrin Amiri, and Hannah Volkman for technical assistance; Andrew Davies for help with DIAS analysis; Jonathan Pierce-Shimomura for help with statistical analysis; and the other members of the McIntire lab for helpful advice. We thank Alan Coulson for cosmids, Yuji Kohara for egl-4 cDNAs, Michael Nonet for sharing strains, the Caenorhabditis Genetics Center for strains, Andy Fire for gfp vector plasmids, Cori Bargmann for various promoter constructs, Takeshi Ishihara and Isao Katsura for che-2 cDNA and H20 promoter constructs, and Stephen Wicks for advice regarding snip-SNP mapping. We also thank Cori Bargmann, Noelle L'Etoile, Takeshi Ishihara, and Isao Katsura for advice and suggestions. Finally, we thank J.A. Lewis and Jonathan Hodgkin for giving us the original inspiration for this work. This work was supported by funds provided by the State of California for medical research on alcohol and substance abuse through the University of California, San Francisco. P.S. was supported in part by the NIH (PO1 NS044232) and the Packard Foundation.
PY - 2002/12/19
Y1 - 2002/12/19
N2 - The growth and behavior of higher organisms depend on the accurate perception and integration of sensory stimuli by the nervous system. We show that defects in sensory perception in C. elegans result in abnormalities in the growth of the animal and in the expression of alternative behavioral states. Our analysis suggests that sensory neurons modulate neural or neuroendocrine functions, regulating both bodily growth and behavioral state. We identify genes likely to be required for these functions downstream of sensory inputs. Here, we characterize one of these genes as egl-4, which we show encodes a cGMP-dependent protein kinase. We demonstrate that this cGMP-dependent kinase functions in neurons of C. elegans to regulate multiple developmental and behavioral processes including the orchestrated growth of the animal and the expression of particular behavioral states.
AB - The growth and behavior of higher organisms depend on the accurate perception and integration of sensory stimuli by the nervous system. We show that defects in sensory perception in C. elegans result in abnormalities in the growth of the animal and in the expression of alternative behavioral states. Our analysis suggests that sensory neurons modulate neural or neuroendocrine functions, regulating both bodily growth and behavioral state. We identify genes likely to be required for these functions downstream of sensory inputs. Here, we characterize one of these genes as egl-4, which we show encodes a cGMP-dependent protein kinase. We demonstrate that this cGMP-dependent kinase functions in neurons of C. elegans to regulate multiple developmental and behavioral processes including the orchestrated growth of the animal and the expression of particular behavioral states.
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U2 - 10.1016/S0896-6273(02)01093-0
DO - 10.1016/S0896-6273(02)01093-0
M3 - Article
C2 - 12495624
AN - SCOPUS:0037137678
VL - 36
SP - 1091
EP - 1102
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 6
ER -