Regulation of expression and trafficking of perforin-2 by LPS and TNF-α

Peng Xiong, Motoaki Shiratsuchi, Takamitsu Matsushima, Jiyuan Liao, Emi Tanaka, Yasuhiro Nakashima, Ryoichi Takayanagi, Yoshihiro Ogawa

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Perforin-2 is constitutively expressed in macrophages that are required for bacterial control. In this study, we found that perforin-2 is expressed in human macrophages with two isoforms: full-length perforin-2a and a splice variant, perforin-2b. Two isoforms show different subcellular distributions. Perforin-2a was predominantly localized to the membrane of endosome-like vesicles by a C-terminal transmembrane domain. In contrast, the short isoform perforin-2b lacking the transmembrane domain failed to localize to the membrane of vesicles. Furthermore, we determined that the pro-inflammatory stimuli LPS and TNF-α induced perforin-2a expression via the NF-κB pathway and triggered perforin-2a vesicles fusion with lysosomes. On the other hand, we detected the secretion of perforin-2b in response to LPS stimulation. Taken together, our data provide the evidence that membrane-bound and secretory isoforms of perforin-2 are present in human macrophages and may play important roles in immune defense.

元の言語英語
ページ(範囲)1-10
ページ数10
ジャーナルCellular Immunology
320
DOI
出版物ステータス出版済み - 10 2017

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Perforin
Protein Isoforms
Macrophages
Membranes
Endosomes
Lysosomes
perforin 2

All Science Journal Classification (ASJC) codes

  • Immunology

これを引用

Regulation of expression and trafficking of perforin-2 by LPS and TNF-α. / Xiong, Peng; Shiratsuchi, Motoaki; Matsushima, Takamitsu; Liao, Jiyuan; Tanaka, Emi; Nakashima, Yasuhiro; Takayanagi, Ryoichi; Ogawa, Yoshihiro.

:: Cellular Immunology, 巻 320, 10.2017, p. 1-10.

研究成果: ジャーナルへの寄稿記事

Xiong, Peng ; Shiratsuchi, Motoaki ; Matsushima, Takamitsu ; Liao, Jiyuan ; Tanaka, Emi ; Nakashima, Yasuhiro ; Takayanagi, Ryoichi ; Ogawa, Yoshihiro. / Regulation of expression and trafficking of perforin-2 by LPS and TNF-α. :: Cellular Immunology. 2017 ; 巻 320. pp. 1-10.
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abstract = "Perforin-2 is constitutively expressed in macrophages that are required for bacterial control. In this study, we found that perforin-2 is expressed in human macrophages with two isoforms: full-length perforin-2a and a splice variant, perforin-2b. Two isoforms show different subcellular distributions. Perforin-2a was predominantly localized to the membrane of endosome-like vesicles by a C-terminal transmembrane domain. In contrast, the short isoform perforin-2b lacking the transmembrane domain failed to localize to the membrane of vesicles. Furthermore, we determined that the pro-inflammatory stimuli LPS and TNF-α induced perforin-2a expression via the NF-κB pathway and triggered perforin-2a vesicles fusion with lysosomes. On the other hand, we detected the secretion of perforin-2b in response to LPS stimulation. Taken together, our data provide the evidence that membrane-bound and secretory isoforms of perforin-2 are present in human macrophages and may play important roles in immune defense.",
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AU - Tanaka, Emi

AU - Nakashima, Yasuhiro

AU - Takayanagi, Ryoichi

AU - Ogawa, Yoshihiro

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