Regulation of expression of the cloned ada gene in Escherichia coli

Yusaku Nakabeppu, Yoshiyuki Mine, Mutsuo Sekiguchi

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

The ada of Escherichia coli K12, the regulatory gene for the adaptive response of bacteria to alkylating agents, was cloned in multicopy plasmids. O6-Methylguanine-DNA methyltransferase and 3-methyladenine-DNA glycosylase II, which are known to be inducible as part of the adaptive response, were produced in ada- cells bearing ada+ plasmids, even without treatment with alkylating agents. When such cells had been treated with methyl methanesulfonate, even higher levels of the enzyme activities were produced. Maxicell experiments revealed that the ada gene codes for a polypeptide with a molecular weight of 38 000. We constructed a hydrid plasmid carrying an ada′-lacZ′ fused gene, with the proper control region for ada expression. ß-Galactosidase synthesis from the fused gene was strongly induced only when cells were treated with low doses of methylating agents, but was weakly induced with relatively high doses of ethylating agents. The induction was autogenously regulated by the ada gene product, in a positive manner.

元の言語英語
ページ(範囲)155-167
ページ数13
ジャーナルMutation Research DNA Repair Reports
146
発行部数2
DOI
出版物ステータス出版済み - 1 1 1985

Fingerprint

Plasmids
Alkylating Agents
Escherichia coli
Galactosidases
Genes
Methyl Methanesulfonate
Escherichia coli K12
Lac Operon
Methyltransferases
Regulator Genes
Molecular Weight
Bacteria
Peptides
DNA
Enzymes
DNA-3-methyladenine glycosidase II
O-(6)-methylguanine

All Science Journal Classification (ASJC) codes

  • Medicine(all)

これを引用

Regulation of expression of the cloned ada gene in Escherichia coli. / Nakabeppu, Yusaku; Mine, Yoshiyuki; Sekiguchi, Mutsuo.

:: Mutation Research DNA Repair Reports, 巻 146, 番号 2, 01.01.1985, p. 155-167.

研究成果: ジャーナルへの寄稿記事

Nakabeppu, Yusaku ; Mine, Yoshiyuki ; Sekiguchi, Mutsuo. / Regulation of expression of the cloned ada gene in Escherichia coli. :: Mutation Research DNA Repair Reports. 1985 ; 巻 146, 番号 2. pp. 155-167.
@article{9dd68adae115456d8cec7e0f6cc1ee68,
title = "Regulation of expression of the cloned ada gene in Escherichia coli",
abstract = "The ada of Escherichia coli K12, the regulatory gene for the adaptive response of bacteria to alkylating agents, was cloned in multicopy plasmids. O6-Methylguanine-DNA methyltransferase and 3-methyladenine-DNA glycosylase II, which are known to be inducible as part of the adaptive response, were produced in ada- cells bearing ada+ plasmids, even without treatment with alkylating agents. When such cells had been treated with methyl methanesulfonate, even higher levels of the enzyme activities were produced. Maxicell experiments revealed that the ada gene codes for a polypeptide with a molecular weight of 38 000. We constructed a hydrid plasmid carrying an ada′-lacZ′ fused gene, with the proper control region for ada expression. {\ss}-Galactosidase synthesis from the fused gene was strongly induced only when cells were treated with low doses of methylating agents, but was weakly induced with relatively high doses of ethylating agents. The induction was autogenously regulated by the ada gene product, in a positive manner.",
author = "Yusaku Nakabeppu and Yoshiyuki Mine and Mutsuo Sekiguchi",
year = "1985",
month = "1",
day = "1",
doi = "10.1016/0167-8817(85)90006-9",
language = "English",
volume = "146",
pages = "155--167",
journal = "Mutation Research DNA Repair Reports",
issn = "0167-8817",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Regulation of expression of the cloned ada gene in Escherichia coli

AU - Nakabeppu, Yusaku

AU - Mine, Yoshiyuki

AU - Sekiguchi, Mutsuo

PY - 1985/1/1

Y1 - 1985/1/1

N2 - The ada of Escherichia coli K12, the regulatory gene for the adaptive response of bacteria to alkylating agents, was cloned in multicopy plasmids. O6-Methylguanine-DNA methyltransferase and 3-methyladenine-DNA glycosylase II, which are known to be inducible as part of the adaptive response, were produced in ada- cells bearing ada+ plasmids, even without treatment with alkylating agents. When such cells had been treated with methyl methanesulfonate, even higher levels of the enzyme activities were produced. Maxicell experiments revealed that the ada gene codes for a polypeptide with a molecular weight of 38 000. We constructed a hydrid plasmid carrying an ada′-lacZ′ fused gene, with the proper control region for ada expression. ß-Galactosidase synthesis from the fused gene was strongly induced only when cells were treated with low doses of methylating agents, but was weakly induced with relatively high doses of ethylating agents. The induction was autogenously regulated by the ada gene product, in a positive manner.

AB - The ada of Escherichia coli K12, the regulatory gene for the adaptive response of bacteria to alkylating agents, was cloned in multicopy plasmids. O6-Methylguanine-DNA methyltransferase and 3-methyladenine-DNA glycosylase II, which are known to be inducible as part of the adaptive response, were produced in ada- cells bearing ada+ plasmids, even without treatment with alkylating agents. When such cells had been treated with methyl methanesulfonate, even higher levels of the enzyme activities were produced. Maxicell experiments revealed that the ada gene codes for a polypeptide with a molecular weight of 38 000. We constructed a hydrid plasmid carrying an ada′-lacZ′ fused gene, with the proper control region for ada expression. ß-Galactosidase synthesis from the fused gene was strongly induced only when cells were treated with low doses of methylating agents, but was weakly induced with relatively high doses of ethylating agents. The induction was autogenously regulated by the ada gene product, in a positive manner.

UR - http://www.scopus.com/inward/record.url?scp=0021932254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021932254&partnerID=8YFLogxK

U2 - 10.1016/0167-8817(85)90006-9

DO - 10.1016/0167-8817(85)90006-9

M3 - Article

C2 - 3929077

AN - SCOPUS:0021932254

VL - 146

SP - 155

EP - 167

JO - Mutation Research DNA Repair Reports

JF - Mutation Research DNA Repair Reports

SN - 0167-8817

IS - 2

ER -