Regulation of expression of the human MTH1 gene encoding 8-Oxo-dGTPase: Alternative splicing of transcription products

Hisanobu Oda, Yusaku Nakabeppu, Masato Furuichi, Mutsuo Sekiguchi

研究成果: Contribution to journalArticle査読

50 被引用数 (Scopus)

抄録

The enzyme 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo- dGTPase) hydrolyzes 8-oxo-dGTP to 8-oxo-dGMP, thereby preventing misincorporation of 8-oxo-dGTP into DNA. We investigated expression of MTH1 gene encoding 8-oxo-dGTPase. Large amounts of MTH1 mRNA were present in thymus and testis, embryonic tissues, and certain cell lines. In peripheral blood lymphocytes, the level of MTH1 mRNA was significantly increased after concomitant treatment with phytohemagglutinin and interleukin-2. Analyses of the 5' regions of the MTH1 transcripts revealed that 7 types of MTH1 mRNAs, which may be produced by transcription initiation at different sites and/or alternative splicing. The MTH1 gene consists of 5 major exons, some of which are composed of differentially processed segments. All types of MTH1 mRNAs carry the entire coding region, and may be functional. Three ATG initiation codons inframe were found in the 5' regions of some of the MTH1 mRNAs. There is a polymorphic alteration at the 5' splicing site (GT to GC) located in exon 2, an event which affects splicing patterns of the MTH1 transcript. Allele frequency of this polymorphism is about 20% among healthy volunteers.

本文言語英語
ページ(範囲)17843-17850
ページ数8
ジャーナルJournal of Biological Chemistry
272
28
DOI
出版ステータス出版済み - 1997

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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