Regulation of matrix metalloproteinase-1 and alpha-smooth muscle actin expression by interleukin-1 alpha and tumour necrosis factor alpha in hepatic stellate cells

Asami Inoue, Kenichi Obayashi, Yuka Sonoda, Anna Nakamura, Takato Ueno, Satoru Kuhara, Kosuke Tashiro

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Hepatic stellate cells (HSCs) are key players in liver fibrosis and regeneration via collagen degradation and synthesis. These phenomena involve inflammatory cytokines released from non-parenchymal liver cells such as Kupffer cells. Although the effects of individual cytokines on many cell types have been investigated in various conditions, such as inflammation and tissue fibrosis, investigating the effect of combined cytokines would further our understanding of the regulatory mechanisms in tissue fibrosis. Here, we report the effect of multiple cytokine combinations on primary HSCs. We first examined the effect of individual cytokines and then the simultaneous exposure of different cytokines, including interleukin-6 (IL-6), IL-1 alpha (IL-1α), platelet-derived growth factor (PDGF), tumour necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β), on matrix metalloproteinase-1 (MMP1) gene expression in primary HSCs. We observed that the combination of all five cytokines induced higher levels of MMP1 gene expression. Of these cytokines, TNF-α and IL-1α were found to be the key cytokines for not only inducing MMP1 expression, but also increasing α-smooth muscle actin gene expression. In conclusion, the combined treatment of TNF-α and IL-1α on HSCs had an enhanced effect on the expression of the fibrotic genes, MMP1 and α-smooth muscle actin, so appears to be an important regulator for tissue regeneration. This finding suggests that stimulation with combined anti-fibrotic cytokines is a potential approach in the development of a novel therapy for the recovery of liver fibrosis.

元の言語英語
ページ(範囲)461-468
ページ数8
ジャーナルCytotechnology
69
発行部数3
DOI
出版物ステータス出版済み - 6 1 2017

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Hepatic Stellate Cells
Matrix Metalloproteinase 1
Interleukin-1alpha
Smooth Muscle
Muscle
Actins
Tumor Necrosis Factor-alpha
Cytokines
Interleukin-1
Gene expression
Liver
Gene Expression
Liver Cirrhosis
Fibrosis
Metalloproteases
Tissue
Tissue regeneration
Liver Regeneration
Kupffer Cells
Platelet-Derived Growth Factor

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

これを引用

Regulation of matrix metalloproteinase-1 and alpha-smooth muscle actin expression by interleukin-1 alpha and tumour necrosis factor alpha in hepatic stellate cells. / Inoue, Asami; Obayashi, Kenichi; Sonoda, Yuka; Nakamura, Anna; Ueno, Takato; Kuhara, Satoru; Tashiro, Kosuke.

:: Cytotechnology, 巻 69, 番号 3, 01.06.2017, p. 461-468.

研究成果: ジャーナルへの寄稿記事

Inoue, Asami ; Obayashi, Kenichi ; Sonoda, Yuka ; Nakamura, Anna ; Ueno, Takato ; Kuhara, Satoru ; Tashiro, Kosuke. / Regulation of matrix metalloproteinase-1 and alpha-smooth muscle actin expression by interleukin-1 alpha and tumour necrosis factor alpha in hepatic stellate cells. :: Cytotechnology. 2017 ; 巻 69, 番号 3. pp. 461-468.
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