抄録
Ad4BP/SF-1 (NR5A1), a member of the nuclear receptor superfamily, is known to play crucial roles in the regulation of steroidogenesis in the gonads and adrenal cortex, and many studies have demonstrated that all steroidogenic genes are direct targets of Ad4BP/SF-1. In addition, in vivo KO studies demonstrated that no steroidogenic organs formed in gene-disrupted mice, strongly suggesting that Ad4BP/SF-1 is essential for the development of the gonads and adrenal gland. However, it remains unclear how Ad4BP/SF-1 regulates the development of the steroidogenic organs and which additional non-steroidogenic genes are targeted. We surveyed the target genes of Ad4BP/SF-1 in steroidogenic cells by mRNA deep sequencing and ChIP-sequence analyses using an Ad4BP/SF-1 antibody. Nearly all genes in energy-producing glycolytic pathways were found to be regulated by Ad4BP/SF-1. We also showed that the key genes implicated in NADPH production are the direct targets. Since sufficient supplies of ATP and NADPH are necessary for cellular survival and production of steroids, Ad4BP/SF-1 possibly orchestrates tissue-specific steroidogenic pathway and these housekeeping metabolic pathways.
| 元の言語 | 英語 |
|---|---|
| ホスト出版物のタイトル | Cell Biology of the Ovary |
| ホスト出版物のサブタイトル | Stem Cells, Development, Cancer, and Clinical Aspects |
| 出版者 | Springer Singapore |
| ページ | 35-43 |
| ページ数 | 9 |
| ISBN（電子版） | 9789811079412 |
| ISBN（印刷物） | 9789811079405 |
| DOI | |
| 出版物ステータス | 出版済み - 6 12 2018 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Medicine(all)
- Health Professions(all)
これを引用
Regulation of metabolic pathways in steroidogenic cells by Ad4BP/SF-1. / Morohashi, Ken Ichirou; Inoue, Miki; Li, Bing; Baba, Takashi.
Cell Biology of the Ovary: Stem Cells, Development, Cancer, and Clinical Aspects. Springer Singapore, 2018. p. 35-43.研究成果: 著書/レポートタイプへの貢献 › 章
}
TY - CHAP
T1 - Regulation of metabolic pathways in steroidogenic cells by Ad4BP/SF-1
AU - Morohashi, Ken Ichirou
AU - Inoue, Miki
AU - Li, Bing
AU - Baba, Takashi
PY - 2018/6/12
Y1 - 2018/6/12
N2 - Ad4BP/SF-1 (NR5A1), a member of the nuclear receptor superfamily, is known to play crucial roles in the regulation of steroidogenesis in the gonads and adrenal cortex, and many studies have demonstrated that all steroidogenic genes are direct targets of Ad4BP/SF-1. In addition, in vivo KO studies demonstrated that no steroidogenic organs formed in gene-disrupted mice, strongly suggesting that Ad4BP/SF-1 is essential for the development of the gonads and adrenal gland. However, it remains unclear how Ad4BP/SF-1 regulates the development of the steroidogenic organs and which additional non-steroidogenic genes are targeted. We surveyed the target genes of Ad4BP/SF-1 in steroidogenic cells by mRNA deep sequencing and ChIP-sequence analyses using an Ad4BP/SF-1 antibody. Nearly all genes in energy-producing glycolytic pathways were found to be regulated by Ad4BP/SF-1. We also showed that the key genes implicated in NADPH production are the direct targets. Since sufficient supplies of ATP and NADPH are necessary for cellular survival and production of steroids, Ad4BP/SF-1 possibly orchestrates tissue-specific steroidogenic pathway and these housekeeping metabolic pathways.
AB - Ad4BP/SF-1 (NR5A1), a member of the nuclear receptor superfamily, is known to play crucial roles in the regulation of steroidogenesis in the gonads and adrenal cortex, and many studies have demonstrated that all steroidogenic genes are direct targets of Ad4BP/SF-1. In addition, in vivo KO studies demonstrated that no steroidogenic organs formed in gene-disrupted mice, strongly suggesting that Ad4BP/SF-1 is essential for the development of the gonads and adrenal gland. However, it remains unclear how Ad4BP/SF-1 regulates the development of the steroidogenic organs and which additional non-steroidogenic genes are targeted. We surveyed the target genes of Ad4BP/SF-1 in steroidogenic cells by mRNA deep sequencing and ChIP-sequence analyses using an Ad4BP/SF-1 antibody. Nearly all genes in energy-producing glycolytic pathways were found to be regulated by Ad4BP/SF-1. We also showed that the key genes implicated in NADPH production are the direct targets. Since sufficient supplies of ATP and NADPH are necessary for cellular survival and production of steroids, Ad4BP/SF-1 possibly orchestrates tissue-specific steroidogenic pathway and these housekeeping metabolic pathways.
UR - http://www.scopus.com/inward/record.url?scp=85053173162&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053173162&partnerID=8YFLogxK
U2 - 10.1007/978-981-10-7941-2_3
DO - 10.1007/978-981-10-7941-2_3
M3 - Chapter
AN - SCOPUS:85053173162
SN - 9789811079405
SP - 35
EP - 43
BT - Cell Biology of the Ovary
PB - Springer Singapore
ER -