TY - JOUR
T1 - Regulation of steroid production
T2 - Analysis of Cyp11a1 promoter
AU - Shih, Meng Chun Monica
AU - Chiu, Yi Ning
AU - Hu, Meng Chun
AU - Guo, Ing Chern
AU - Chung, Bon chu
N1 - Funding Information:
We would like to thank Shu-Jane Chou for technical assistance. This work was supported by grants from Academia Sinica, National Health Research Institute ( NHRI-EX99-9710SI ) and from National Science Council ( NSC 99-2321-B-001-009 and NSC97-2320-B-002-041-MY3 ), Taiwan.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/4/10
Y1 - 2011/4/10
N2 - CYP11A1 is a key enzyme in steroid synthesis abundantly expressed in the adrenal, testis, ovary, and placenta. This article reviews recent studies on cis-regulatory elements and trans-regulators of the CYP11A1 promoter, with special focus on their tissue-specific regulation. Trans-regulators include tissue-specific factors such as SF-1, DAX-1, TReP-132, LBP, and GATA that regulate tissue-specific expression of CYP11A1. These tissue-specific factors interact with factors commonly present in most cells like AP-1, Sp1, and AP-2 to bring CYP11A1 transcription to full potential. These transcription factors stimulate CYP11A1 transcriptional activity through interaction with their specific cis-elements or through protein-protein interaction. The cis-element on the Cyp11a1 promoter was further characterized in vitro and in vivo. Mutation of the proximal SF-1-binding site results in down regulation of CYP11A1 in the adrenal and testis but not in the ovary and placenta, leading to attenuated corticosterone circadian rhythms and blunted stress response.
AB - CYP11A1 is a key enzyme in steroid synthesis abundantly expressed in the adrenal, testis, ovary, and placenta. This article reviews recent studies on cis-regulatory elements and trans-regulators of the CYP11A1 promoter, with special focus on their tissue-specific regulation. Trans-regulators include tissue-specific factors such as SF-1, DAX-1, TReP-132, LBP, and GATA that regulate tissue-specific expression of CYP11A1. These tissue-specific factors interact with factors commonly present in most cells like AP-1, Sp1, and AP-2 to bring CYP11A1 transcription to full potential. These transcription factors stimulate CYP11A1 transcriptional activity through interaction with their specific cis-elements or through protein-protein interaction. The cis-element on the Cyp11a1 promoter was further characterized in vitro and in vivo. Mutation of the proximal SF-1-binding site results in down regulation of CYP11A1 in the adrenal and testis but not in the ovary and placenta, leading to attenuated corticosterone circadian rhythms and blunted stress response.
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U2 - 10.1016/j.mce.2010.12.017
DO - 10.1016/j.mce.2010.12.017
M3 - Review article
C2 - 21195129
AN - SCOPUS:79952067865
SN - 0303-7207
VL - 336
SP - 80
EP - 84
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -