Relationship between chemically induced cell damage and aryl hydrocarbon hydroxylase activity in several lymphoblastoid cell lines

Chikako Kiyohara, M. Washio, T. Hirohata

研究成果: ジャーナルへの寄稿記事

抄録

To clarify the relationship between chemically-induced cell damage and aryl hydrocarbon hydroxylase (AHH) activity, we used five lines from ataxia telangiectasia (AT) homozygotes (n = 5), AT heterozygotes (n = 5), xeroderma pigmentosum (XP) homozygotes (n = 2), XP heterozygotes (n = 2), and healthy subjects (n = 54). The value of relative fluorescein formation (RFF; an index of cell damage) of cell lines from either AT or XP homozygotes was significantly lower (p < 0.01 and p < 0.001, respectively) than that of lines from healthy subjects. The value of neocarzinostatin (NCS)-treated RFF of cell lines from XP homozygotes, which are sensitive to UV or UV-mimetic chemicals, was also significantly lower than that of healthy subjects (p < 0.05) and similar to that of AT heterozygotes. Since both XP and AT patients have increased risks of internal neoplasms, these might be related to exposure to environmental carcinogens. Although high levels of AHH activity may indicate more conversion of carcinogens into highly reactive intermediates, which interact with DNA to cause carcinogenicity, AHH activity showed no significant difference among these five cell lines. The differences in endogenous activation ability may not be a large factor in the hypersensitivity of XP and AT patients to environmental carcinogens.

元の言語英語
ページ(範囲)611-614
ページ数4
ジャーナルMedical Science Research
25
発行部数9
出版物ステータス出版済み - 10 30 1997

Fingerprint

Aryl Hydrocarbon Hydroxylases
Xeroderma Pigmentosum
Ataxia Telangiectasia
Cells
Homozygote
Cell Line
Environmental Carcinogens
Heterozygote
Healthy Volunteers
Zinostatin
Fluorescein
Carcinogens
Chemical activation
Hypersensitivity
DNA
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Relationship between chemically induced cell damage and aryl hydrocarbon hydroxylase activity in several lymphoblastoid cell lines. / Kiyohara, Chikako; Washio, M.; Hirohata, T.

:: Medical Science Research, 巻 25, 番号 9, 30.10.1997, p. 611-614.

研究成果: ジャーナルへの寄稿記事

@article{42b87fb93f9741d6a16de9569de62e87,
title = "Relationship between chemically induced cell damage and aryl hydrocarbon hydroxylase activity in several lymphoblastoid cell lines",
abstract = "To clarify the relationship between chemically-induced cell damage and aryl hydrocarbon hydroxylase (AHH) activity, we used five lines from ataxia telangiectasia (AT) homozygotes (n = 5), AT heterozygotes (n = 5), xeroderma pigmentosum (XP) homozygotes (n = 2), XP heterozygotes (n = 2), and healthy subjects (n = 54). The value of relative fluorescein formation (RFF; an index of cell damage) of cell lines from either AT or XP homozygotes was significantly lower (p < 0.01 and p < 0.001, respectively) than that of lines from healthy subjects. The value of neocarzinostatin (NCS)-treated RFF of cell lines from XP homozygotes, which are sensitive to UV or UV-mimetic chemicals, was also significantly lower than that of healthy subjects (p < 0.05) and similar to that of AT heterozygotes. Since both XP and AT patients have increased risks of internal neoplasms, these might be related to exposure to environmental carcinogens. Although high levels of AHH activity may indicate more conversion of carcinogens into highly reactive intermediates, which interact with DNA to cause carcinogenicity, AHH activity showed no significant difference among these five cell lines. The differences in endogenous activation ability may not be a large factor in the hypersensitivity of XP and AT patients to environmental carcinogens.",
author = "Chikako Kiyohara and M. Washio and T. Hirohata",
year = "1997",
month = "10",
day = "30",
language = "English",
volume = "25",
pages = "611--614",
journal = "Medical Science Research",
issn = "0269-8951",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - Relationship between chemically induced cell damage and aryl hydrocarbon hydroxylase activity in several lymphoblastoid cell lines

AU - Kiyohara, Chikako

AU - Washio, M.

AU - Hirohata, T.

PY - 1997/10/30

Y1 - 1997/10/30

N2 - To clarify the relationship between chemically-induced cell damage and aryl hydrocarbon hydroxylase (AHH) activity, we used five lines from ataxia telangiectasia (AT) homozygotes (n = 5), AT heterozygotes (n = 5), xeroderma pigmentosum (XP) homozygotes (n = 2), XP heterozygotes (n = 2), and healthy subjects (n = 54). The value of relative fluorescein formation (RFF; an index of cell damage) of cell lines from either AT or XP homozygotes was significantly lower (p < 0.01 and p < 0.001, respectively) than that of lines from healthy subjects. The value of neocarzinostatin (NCS)-treated RFF of cell lines from XP homozygotes, which are sensitive to UV or UV-mimetic chemicals, was also significantly lower than that of healthy subjects (p < 0.05) and similar to that of AT heterozygotes. Since both XP and AT patients have increased risks of internal neoplasms, these might be related to exposure to environmental carcinogens. Although high levels of AHH activity may indicate more conversion of carcinogens into highly reactive intermediates, which interact with DNA to cause carcinogenicity, AHH activity showed no significant difference among these five cell lines. The differences in endogenous activation ability may not be a large factor in the hypersensitivity of XP and AT patients to environmental carcinogens.

AB - To clarify the relationship between chemically-induced cell damage and aryl hydrocarbon hydroxylase (AHH) activity, we used five lines from ataxia telangiectasia (AT) homozygotes (n = 5), AT heterozygotes (n = 5), xeroderma pigmentosum (XP) homozygotes (n = 2), XP heterozygotes (n = 2), and healthy subjects (n = 54). The value of relative fluorescein formation (RFF; an index of cell damage) of cell lines from either AT or XP homozygotes was significantly lower (p < 0.01 and p < 0.001, respectively) than that of lines from healthy subjects. The value of neocarzinostatin (NCS)-treated RFF of cell lines from XP homozygotes, which are sensitive to UV or UV-mimetic chemicals, was also significantly lower than that of healthy subjects (p < 0.05) and similar to that of AT heterozygotes. Since both XP and AT patients have increased risks of internal neoplasms, these might be related to exposure to environmental carcinogens. Although high levels of AHH activity may indicate more conversion of carcinogens into highly reactive intermediates, which interact with DNA to cause carcinogenicity, AHH activity showed no significant difference among these five cell lines. The differences in endogenous activation ability may not be a large factor in the hypersensitivity of XP and AT patients to environmental carcinogens.

UR - http://www.scopus.com/inward/record.url?scp=0030762303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030762303&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 611

EP - 614

JO - Medical Science Research

JF - Medical Science Research

SN - 0269-8951

IS - 9

ER -