抄録
To clarify the relationship between chemically-induced cell damage and aryl hydrocarbon hydroxylase (AHH) activity, we used five lines from ataxia telangiectasia (AT) homozygotes (n = 5), AT heterozygotes (n = 5), xeroderma pigmentosum (XP) homozygotes (n = 2), XP heterozygotes (n = 2), and healthy subjects (n = 54). The value of relative fluorescein formation (RFF; an index of cell damage) of cell lines from either AT or XP homozygotes was significantly lower (p < 0.01 and p < 0.001, respectively) than that of lines from healthy subjects. The value of neocarzinostatin (NCS)-treated RFF of cell lines from XP homozygotes, which are sensitive to UV or UV-mimetic chemicals, was also significantly lower than that of healthy subjects (p < 0.05) and similar to that of AT heterozygotes. Since both XP and AT patients have increased risks of internal neoplasms, these might be related to exposure to environmental carcinogens. Although high levels of AHH activity may indicate more conversion of carcinogens into highly reactive intermediates, which interact with DNA to cause carcinogenicity, AHH activity showed no significant difference among these five cell lines. The differences in endogenous activation ability may not be a large factor in the hypersensitivity of XP and AT patients to environmental carcinogens.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 611-614 |
| ページ数 | 4 |
| ジャーナル | Medical Science Research |
| 巻 | 25 |
| 発行部数 | 9 |
| 出版物ステータス | 出版済み - 10 30 1997 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
これを引用
Relationship between chemically induced cell damage and aryl hydrocarbon hydroxylase activity in several lymphoblastoid cell lines. / Kiyohara, Chikako; Washio, M.; Hirohata, T.
：: Medical Science Research, 巻 25, 番号 9, 30.10.1997, p. 611-614.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Relationship between chemically induced cell damage and aryl hydrocarbon hydroxylase activity in several lymphoblastoid cell lines
AU - Kiyohara, Chikako
AU - Washio, M.
AU - Hirohata, T.
PY - 1997/10/30
Y1 - 1997/10/30
N2 - To clarify the relationship between chemically-induced cell damage and aryl hydrocarbon hydroxylase (AHH) activity, we used five lines from ataxia telangiectasia (AT) homozygotes (n = 5), AT heterozygotes (n = 5), xeroderma pigmentosum (XP) homozygotes (n = 2), XP heterozygotes (n = 2), and healthy subjects (n = 54). The value of relative fluorescein formation (RFF; an index of cell damage) of cell lines from either AT or XP homozygotes was significantly lower (p < 0.01 and p < 0.001, respectively) than that of lines from healthy subjects. The value of neocarzinostatin (NCS)-treated RFF of cell lines from XP homozygotes, which are sensitive to UV or UV-mimetic chemicals, was also significantly lower than that of healthy subjects (p < 0.05) and similar to that of AT heterozygotes. Since both XP and AT patients have increased risks of internal neoplasms, these might be related to exposure to environmental carcinogens. Although high levels of AHH activity may indicate more conversion of carcinogens into highly reactive intermediates, which interact with DNA to cause carcinogenicity, AHH activity showed no significant difference among these five cell lines. The differences in endogenous activation ability may not be a large factor in the hypersensitivity of XP and AT patients to environmental carcinogens.
AB - To clarify the relationship between chemically-induced cell damage and aryl hydrocarbon hydroxylase (AHH) activity, we used five lines from ataxia telangiectasia (AT) homozygotes (n = 5), AT heterozygotes (n = 5), xeroderma pigmentosum (XP) homozygotes (n = 2), XP heterozygotes (n = 2), and healthy subjects (n = 54). The value of relative fluorescein formation (RFF; an index of cell damage) of cell lines from either AT or XP homozygotes was significantly lower (p < 0.01 and p < 0.001, respectively) than that of lines from healthy subjects. The value of neocarzinostatin (NCS)-treated RFF of cell lines from XP homozygotes, which are sensitive to UV or UV-mimetic chemicals, was also significantly lower than that of healthy subjects (p < 0.05) and similar to that of AT heterozygotes. Since both XP and AT patients have increased risks of internal neoplasms, these might be related to exposure to environmental carcinogens. Although high levels of AHH activity may indicate more conversion of carcinogens into highly reactive intermediates, which interact with DNA to cause carcinogenicity, AHH activity showed no significant difference among these five cell lines. The differences in endogenous activation ability may not be a large factor in the hypersensitivity of XP and AT patients to environmental carcinogens.
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M3 - Article
VL - 25
SP - 611
EP - 614
JO - Medical Science Research
JF - Medical Science Research
SN - 0269-8951
IS - 9
ER -