Relationship between chemically induced cell damage and aryl hydrocarbon hydroxylase activity in several lymphoblastoid cell lines

C. Kiyohara, M. Washio, T. Hirohata

研究成果: ジャーナルへの寄稿学術誌査読

抄録

To clarify the relationship between chemically-induced cell damage and aryl hydrocarbon hydroxylase (AHH) activity, we used five lines from ataxia telangiectasia (AT) homozygotes (n = 5), AT heterozygotes (n = 5), xeroderma pigmentosum (XP) homozygotes (n = 2), XP heterozygotes (n = 2), and healthy subjects (n = 54). The value of relative fluorescein formation (RFF; an index of cell damage) of cell lines from either AT or XP homozygotes was significantly lower (p < 0.01 and p < 0.001, respectively) than that of lines from healthy subjects. The value of neocarzinostatin (NCS)-treated RFF of cell lines from XP homozygotes, which are sensitive to UV or UV-mimetic chemicals, was also significantly lower than that of healthy subjects (p < 0.05) and similar to that of AT heterozygotes. Since both XP and AT patients have increased risks of internal neoplasms, these might be related to exposure to environmental carcinogens. Although high levels of AHH activity may indicate more conversion of carcinogens into highly reactive intermediates, which interact with DNA to cause carcinogenicity, AHH activity showed no significant difference among these five cell lines. The differences in endogenous activation ability may not be a large factor in the hypersensitivity of XP and AT patients to environmental carcinogens.

本文言語英語
ページ(範囲)611-614
ページ数4
ジャーナルMedical Science Research
25
9
出版ステータス出版済み - 10月 30 1997

!!!All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「Relationship between chemically induced cell damage and aryl hydrocarbon hydroxylase activity in several lymphoblastoid cell lines」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル