Relaxation of the carotid artery to hypoxia is impaired in Watanabe heritable hyperlipidemic rabbits

Hisao Taguchi, Frank M. Faraci, Takanari Kitazono, Donald D. Heistad

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

We tested the hypothesis that relaxation of the carotid artery during hypoxia is mediated by activation of glibenclamide-sensitive potassium channels and that this response is impaired in hyperlipidemic rabbits. In New Zealand White rabbits (plasma cholesterol, 69±12 mg/dL, mean±SEM) and Watanabe heritable hyperlipidemic (WHHL) rabbits (plasma cholesterol, 677±99 mg/dL), tension of the carotid artery was measured in an organ bath under control conditions and during two levels of hypoxia. In normal rabbits, mild hypoxia produced 21±2% relaxation in arteries precontracted with phenylephrine. Removal of endothelium or the nitric oxide synthase inhibitor N(G)-nitro-L-arginine (10-4 mol/L) almost abolished relaxation in response to mild hypoxia in normal rabbits. Glibenclamide (10-6 mol/L), an inhibitor of ATP-sensitive potassium channels, attenuated relaxation during mild hypoxia by almost 60%. In WHHL rabbits mild hypoxia relaxed the carotid artery by only 9±4% (P<.05 versus normal rabbits). Severe hypoxia produced greater relaxation of the carotid artery in normal than in WHHL rabbits (85±5% versus 52±8%, respectively, P<.05). Glibenclamide but not endothelial denudation or N(G)-nitro-L-arginine attenuated relaxation during severe hypoxia in normal and WHHL rabbits. Relaxation of the carotid artery to sodium nitroprusside was similar in normal and WHHL rabbits. These findings suggest that relaxation of the carotid artery in response to mild and severe hypoxia is impaired in WHHL rabbits and is mediated, in large part, by activation of glibenclamide-sensitive potassium channels. Relaxation of the carotid artery in response to mild hypoxia is mediated primarily by endothelium-derived relaxing factor in normal rabbits and impairment of response to mild hypoxia in WHHL is probably secondary to endothelial dysfunction.

元の言語英語
ページ(範囲)1641-1645
ページ数5
ジャーナルArteriosclerosis, thrombosis, and vascular biology
15
発行部数10
DOI
出版物ステータス出版済み - 1 1 1995
外部発表Yes

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Carotid Arteries
Rabbits
Glyburide
Potassium Channels
Arginine
Hypoxia
Cholesterol
Endothelium-Dependent Relaxing Factors
KATP Channels
Nitroprusside
Phenylephrine
Baths
Nitric Oxide Synthase
Endothelium
Arteries

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

これを引用

Relaxation of the carotid artery to hypoxia is impaired in Watanabe heritable hyperlipidemic rabbits. / Taguchi, Hisao; Faraci, Frank M.; Kitazono, Takanari; Heistad, Donald D.

:: Arteriosclerosis, thrombosis, and vascular biology, 巻 15, 番号 10, 01.01.1995, p. 1641-1645.

研究成果: ジャーナルへの寄稿記事

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title = "Relaxation of the carotid artery to hypoxia is impaired in Watanabe heritable hyperlipidemic rabbits",
abstract = "We tested the hypothesis that relaxation of the carotid artery during hypoxia is mediated by activation of glibenclamide-sensitive potassium channels and that this response is impaired in hyperlipidemic rabbits. In New Zealand White rabbits (plasma cholesterol, 69±12 mg/dL, mean±SEM) and Watanabe heritable hyperlipidemic (WHHL) rabbits (plasma cholesterol, 677±99 mg/dL), tension of the carotid artery was measured in an organ bath under control conditions and during two levels of hypoxia. In normal rabbits, mild hypoxia produced 21±2{\%} relaxation in arteries precontracted with phenylephrine. Removal of endothelium or the nitric oxide synthase inhibitor N(G)-nitro-L-arginine (10-4 mol/L) almost abolished relaxation in response to mild hypoxia in normal rabbits. Glibenclamide (10-6 mol/L), an inhibitor of ATP-sensitive potassium channels, attenuated relaxation during mild hypoxia by almost 60{\%}. In WHHL rabbits mild hypoxia relaxed the carotid artery by only 9±4{\%} (P<.05 versus normal rabbits). Severe hypoxia produced greater relaxation of the carotid artery in normal than in WHHL rabbits (85±5{\%} versus 52±8{\%}, respectively, P<.05). Glibenclamide but not endothelial denudation or N(G)-nitro-L-arginine attenuated relaxation during severe hypoxia in normal and WHHL rabbits. Relaxation of the carotid artery to sodium nitroprusside was similar in normal and WHHL rabbits. These findings suggest that relaxation of the carotid artery in response to mild and severe hypoxia is impaired in WHHL rabbits and is mediated, in large part, by activation of glibenclamide-sensitive potassium channels. Relaxation of the carotid artery in response to mild hypoxia is mediated primarily by endothelium-derived relaxing factor in normal rabbits and impairment of response to mild hypoxia in WHHL is probably secondary to endothelial dysfunction.",
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AU - Heistad, Donald D.

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AB - We tested the hypothesis that relaxation of the carotid artery during hypoxia is mediated by activation of glibenclamide-sensitive potassium channels and that this response is impaired in hyperlipidemic rabbits. In New Zealand White rabbits (plasma cholesterol, 69±12 mg/dL, mean±SEM) and Watanabe heritable hyperlipidemic (WHHL) rabbits (plasma cholesterol, 677±99 mg/dL), tension of the carotid artery was measured in an organ bath under control conditions and during two levels of hypoxia. In normal rabbits, mild hypoxia produced 21±2% relaxation in arteries precontracted with phenylephrine. Removal of endothelium or the nitric oxide synthase inhibitor N(G)-nitro-L-arginine (10-4 mol/L) almost abolished relaxation in response to mild hypoxia in normal rabbits. Glibenclamide (10-6 mol/L), an inhibitor of ATP-sensitive potassium channels, attenuated relaxation during mild hypoxia by almost 60%. In WHHL rabbits mild hypoxia relaxed the carotid artery by only 9±4% (P<.05 versus normal rabbits). Severe hypoxia produced greater relaxation of the carotid artery in normal than in WHHL rabbits (85±5% versus 52±8%, respectively, P<.05). Glibenclamide but not endothelial denudation or N(G)-nitro-L-arginine attenuated relaxation during severe hypoxia in normal and WHHL rabbits. Relaxation of the carotid artery to sodium nitroprusside was similar in normal and WHHL rabbits. These findings suggest that relaxation of the carotid artery in response to mild and severe hypoxia is impaired in WHHL rabbits and is mediated, in large part, by activation of glibenclamide-sensitive potassium channels. Relaxation of the carotid artery in response to mild hypoxia is mediated primarily by endothelium-derived relaxing factor in normal rabbits and impairment of response to mild hypoxia in WHHL is probably secondary to endothelial dysfunction.

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