Release of Ca 2+ from a non-mitochondrial store site in peritoneal macrophages treated with saponin by inositol 1,4,5-trisphosphate

Masato Hirata, E. Suematsu, T. Hashimoto

研究成果: ジャーナルへの寄稿記事

111 引用 (Scopus)

抄録

The effects of inositol 1,4,5-trisphosphate, prepared from human erythrocyte ghosts, on Ca 2+ release from intracellular store sites were studied in saponin-treated guinea pig peritoneal macrophages. Micromolar concentrations of inositol 1,4,5-trisphosphate released Ca 2+ within 1 min from store sites which had accumulated Ca 2+ in the presence of 10 mM-NaN 3 . In the presence of 10 mM-NaN 3 , the Ca 2+ accumulated in the presence of oxalate was seen in the endoplasmic reticulum of saponin-treated macrophages by electron microscopy, indicating that the site of Ca 2+ released by inositol 1,4,5-trisphophate may be endoplasmic reticulum-like membranes. When the concentrations of free Ca 2+ were over 3.5x10 -6 M, the release of Ca 2+ by this agent was inhibited. This inhibition may be due to either the higher concentration of extra-vesicular free Ca 2+ or the larger accumulation of Ca 2+ into the store site or perhaps both effects. MgCl 2 also had an inhibitory effect on the Ca 2+ release. Inositol 1,4,5-trisphosphate also released Ca 2+ from cardiac sarcoplasmic reticulum, but not from erythrocyte inside-out vesicles.

元の言語英語
ページ(範囲)229-236
ページ数8
ジャーナルBiochemical Journal
223
発行部数1
DOI
出版物ステータス出版済み - 1 1 1984

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Inositol 1,4,5-Trisphosphate
Macrophages
Saponins
Peritoneal Macrophages
Endoplasmic Reticulum
Oxalates
Erythrocyte Membrane
Sarcoplasmic Reticulum
Inositol
Electron microscopy
Electron Microscopy
Guinea Pigs
Erythrocytes
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Release of Ca 2+ from a non-mitochondrial store site in peritoneal macrophages treated with saponin by inositol 1,4,5-trisphosphate . / Hirata, Masato; Suematsu, E.; Hashimoto, T.

:: Biochemical Journal, 巻 223, 番号 1, 01.01.1984, p. 229-236.

研究成果: ジャーナルへの寄稿記事

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abstract = "The effects of inositol 1,4,5-trisphosphate, prepared from human erythrocyte ghosts, on Ca 2+ release from intracellular store sites were studied in saponin-treated guinea pig peritoneal macrophages. Micromolar concentrations of inositol 1,4,5-trisphosphate released Ca 2+ within 1 min from store sites which had accumulated Ca 2+ in the presence of 10 mM-NaN 3 . In the presence of 10 mM-NaN 3 , the Ca 2+ accumulated in the presence of oxalate was seen in the endoplasmic reticulum of saponin-treated macrophages by electron microscopy, indicating that the site of Ca 2+ released by inositol 1,4,5-trisphophate may be endoplasmic reticulum-like membranes. When the concentrations of free Ca 2+ were over 3.5x10 -6 M, the release of Ca 2+ by this agent was inhibited. This inhibition may be due to either the higher concentration of extra-vesicular free Ca 2+ or the larger accumulation of Ca 2+ into the store site or perhaps both effects. MgCl 2 also had an inhibitory effect on the Ca 2+ release. Inositol 1,4,5-trisphosphate also released Ca 2+ from cardiac sarcoplasmic reticulum, but not from erythrocyte inside-out vesicles.",
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