Relocalization of apoptosis-inducing factor in photoreceptor apoptosis induced by retinal detachment in vivo

Toshio Hisatomi, Taiji Sakamoto, Toshinori Murata, Ichiro Yamanaka, Yuji Oshima, Yasuaki Hata, Tatsuro Ishibashi, Hajime Inomata, Santos A. Susin, Guido Kroemer

研究成果: Contribution to journalArticle査読

148 被引用数 (Scopus)


Apoptosis-inducing factor (AIF) is a novel mediator in apoptosis. AIF is a flavoprotein that is normally confined to the mitochondrial intermembrane space, yet translocates to the nucleus in several in vitro models of apoptosis. To investigate the role of AIF in the apoptotic process in vivo, we induced retinal detachment (RD) by subretinal injection of sodium hyaluronate, either in Brown Norway rats or in C3H mice. Apoptotic DNA fragmentation, as determined by terminal nick-end labeling, was most prominent 3 days after RD. The subcellular localization of AIF was examined by immunohistochemistry and immunoelectron microscopy. In normal photoreceptor cells, AIF was present in the mitochondrion-rich inner segment. However, AIF was found in the nucleus after RD. Photoreceptor apoptosis developed similarly in C3H control mice, and in mice bearing the gld or lpr mutations, indicating that cell death occurs independently from the CD95/CD95 ligand system. Both the mitochondrio-nuclear transition of AIF localization and the nuclear DNA fragmentation were inhibited by subretinal application of brain-derived neurotrophic factor. To our knowledge, this is the first description of AIF relocalization occurring in a clinically relevant, in vivo model of apoptosis.

ジャーナルAmerican Journal of Pathology
出版ステータス出版済み - 2001

All Science Journal Classification (ASJC) codes

  • 病理学および法医学


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