Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins

Mitsunori Kato, Keiji Kito, Kazuhisa Ota, Takashi Ito

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Ubiquitination regulates not only the stability but the localization and activity of substrate proteins involved in a plethora of cellular processes. The Skp1-Cullin-F-box protein (SCF) complexes constitute a major family of ubiquitin protein ligases, in each member of which an F-box protein serves as the variable component responsible for substrate recognition, thereby defining the function of each complex. Here we studied whether the composition of F-box proteins in the SCF complexes is remodeled under different conditions. We exploited stable isotope labeling and MS for relative quantification of F-box proteins in the SCF complexes affinity-purified en masse from budding yeast cells at log and post-diauxic phases, and revealed an increment of Saf1, an F-box protein involved in entry into quiescence, during the diauxic shift. Similarly, we found that Met4 overexpression induces a specific increment of Met30, the F-box protein responsible for ubiquitination of Met4. These results illustrate a cellular response to environmental and genetic perturbations through remodeling of the SCF complex-mediated ubiquitination system. Compositional alteration of incorporated F-box proteins may redirect the activity of this system toward appropriate substrates to be ubiquitinated under individual conditions for the maintenance of cellular homeostasis.

元の言語英語
ページ(範囲)115-123
ページ数9
ジャーナルProteomics
10
発行部数1
DOI
出版物ステータス出版済み - 1 1 2010
外部発表Yes

Fingerprint

SKP Cullin F-Box Protein Ligases
F-Box Proteins
Ubiquitination
Substrates
Isotope Labeling
Saccharomycetales
Ubiquitin-Protein Ligases
Isotopes
Yeast
Labeling
Homeostasis
Cells
Maintenance
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry

これを引用

Remodeling of the SCF complex-mediated ubiquitination system by compositional alteration of incorporated F-box proteins. / Kato, Mitsunori; Kito, Keiji; Ota, Kazuhisa; Ito, Takashi.

:: Proteomics, 巻 10, 番号 1, 01.01.2010, p. 115-123.

研究成果: ジャーナルへの寄稿記事

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