Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-specific gene integration system

Yoshinori Kawabe, Hirokatsu Makitsubo, Yujiro Kameyama, Shuohao Huang, Akira Ito, Masamichi Kamihira

研究成果: Contribution to journalArticle査読

28 被引用数 (Scopus)

抄録

We previously reported an accumulative site-specific gene integration system using Cre recombinase and mutated loxP sites, where a recombinasemediated cassette exchange (RMCE) reaction is repeatable. This gene integration system was applied for antibody production using recombinant Chinese hamster ovary (CHO) cells. We introduced an exchange cassette flanked by wild-type and mutated loxP sites into the chromosome of CHO cells for the establishment of recipient founder cells. Then, the donor plasmids including an expression cassette for an antibody gene flanked by a compatible pair of loxP sites were prepared. The donor plasmid and a Cre expression vector were co-transfected into the founder CHO cells to give rise to RMCE in the CHO genome, resulting in site-specific integration of the antibody gene. The RMCE procedure was repeated to increase the copy numbers of the integrated gene. Southern blot and genomic PCR analyses for the established cells revealed that the transgenes were integrated into the target site. Antibody production determined by ELISA and western blotting was increased corresponding to the number of transgenes. These results indicate that the accumulative site-specific gene integration system could provide a useful tool for increasing the productivity of recombinant proteins.

本文言語英語
ページ(範囲)267-279
ページ数13
ジャーナルCytotechnology
64
3
DOI
出版ステータス出版済み - 5 2012

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体医工学
  • 臨床生化学
  • 細胞生物学

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