Replication factors required for SV40 DNA replication in vitro. II. Switching of DNA polymerase α and δ during initiation of leading and lagging strand synthesis

T. Tsurimoto, B. Stillman

研究成果: ジャーナルへの寄稿学術誌査読

213 被引用数 (Scopus)

抄録

Replication factors A and C (RF-A and RF-C) and the proliferating cell nuclear antigen (PCNA) differentially augment the activities of DNA polymerases α and δ. The mechanism of stimulation by these replication factors was investigated using a limiting concentration of primed, single-stranded template DNA. RF-A stimulated polymerase α activity in a concentration-dependent manner, but also suppressed nonspecific initiation of DNA synthesis by both polymerases α and δ. The primer recognition complex, RF-C·PCNA·ATP, stimulated pol δ activity in cooperation with RF-A, but also functioned to prevent abnormal initiation of DNA synthesis by polymerase α. Reconstitution of DNA replication with purified factors and a plasmid containing the SV40 origin sequences directly demonstrated DNA polymerase α dependent synthesis of lagging strands and DNA polymerase δ/PCNA/RF-C dependent synthesis of leading strands. RF-A and the primer recognition complex both affected the relative levels of leading and lagging strands. These results, in addition to results in an accompanying paper (Tsurimoto,T., and Stillman, B. (1991) J. Biol. Chem. 266, 1950-1960), suggest that an exchange of DNA polymerase complexes occurs during initiation of bidirectional DNA replication at the SV40 origin.

本文言語英語
ページ(範囲)1961-1968
ページ数8
ジャーナルJournal of Biological Chemistry
266
3
出版ステータス出版済み - 1991
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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