Reproductive toxicity of ethylene glycol monoethyl ether in Aldh2 knockout mice

Rui Sheng Wang, Katsumi Ohtani, Megumi Suda, Kyoko Kitagawa, Keiichi Nakayama, Toshihiro Kawamoto, Tamie Nakajima

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)


Ethylene glycol monoethyl ether (EGEE) can cause damage to testes and sperm, and its metabolites are believed to play an important role in its toxicity. Aldehyde dehydrogenase 2 (ALDH2) is involved in the metabolism of this chemical. To investigate whether and how the enzyme affects the toxicity of EGEE, we conducted experiments comparing Aldh2 knockout mice with wild-type mice. Administration of EGEE at 100 and 600 mg/kg/day for one week did not induce any significant change in the weight and body weight ratios of testes, prostate and epididymides in either Aldh2 knockout or wild-type mice. However, motion of sperm from the spermaduct, as analyzed with a Hamilton-Thorne Sperm analyzer, was slightly decreased in the low dose group, and significantly lower in the high dose group; and the percentage of progressive sperm was also reduced in the two EGEE groups. This effect of EGEE treatment was observed in the wild-type, but not in the Aldh2 knockout mice. Sperm motion from the cauda epididymides was not affected. On the other hand, the concentration of ethoxyacetic acid, a metabolite of EGEE, in 24 h pooled urine of EGEE-treated Aldh2 knockout mice was not significantly lower than that of the wild-type mice on most days of urine sampling. These results suggest that inactivation of the ALDH2 enzyme due to gene mutation may be linked to differences in the susceptibility to EGEE-induced sperm toxicity.

ジャーナルIndustrial health
出版ステータス出版済み - 8月 2007

!!!All Science Journal Classification (ASJC) codes

  • 公衆衛生学、環境および労働衛生
  • 健康、毒物学および変異誘発


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