Reprogramming of mouse and human cells to pluripotency using mature microRNAs

Norikatsu Miyoshi, Hideshi Ishii, Hiroaki Nagano, Naotsugu Haraguchi, Dyah Laksmi Dewi, Yoshihiro Kano, Shinpei Nishikawa, Masahiro Tanemura, Koshi Mimori, Fumiaki Tanaka, Toshiyuki Saito, Junichi Nishimura, Ichiro Takemasa, Tsunekazu Mizushima, Masataka Ikeda, Hirofumi Yamamoto, Mitsugu Sekimoto, Yuichiro Doki, Masaki Mori

研究成果: Contribution to journalArticle査読

544 被引用数 (Scopus)


Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.

ジャーナルCell stem cell
出版ステータス出版済み - 6 3 2011

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Genetics
  • Cell Biology

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