Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

Hiroaki Nagatomo, Nanami Kohri, Hiroki Akizawa, Yumi Hoshino, Nobuhiko Yamauchi, Tomohiro Kono, Masashi Takahashi, Manabu Kawahara

研究成果: Contribution to journalArticle

4 引用 (Scopus)

抜粋

Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.

元の言語英語
ページ(範囲)457-461
ページ数5
ジャーナルAnimal Science Journal
87
発行部数3
DOI
出版物ステータス出版済み - 3 1 2016

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

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    Nagatomo, H., Kohri, N., Akizawa, H., Hoshino, Y., Yamauchi, N., Kono, T., Takahashi, M., & Kawahara, M. (2016). Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development. Animal Science Journal, 87(3), 457-461. https://doi.org/10.1111/asj.12538