Resistance to influenza A virus infection in transformed cell lines expressing an anti-PB2 monoclonal antibody

Yoshikazu Fujimoto, Kinuyo Ozaki, Masahiro Maeda, Ken ichi Nishijima, Hiroki Takakuwa, Koichi Otsuki, Hiroshi Kida, Etsuro Ono

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

The polymerase basic 2 (PB2) protein is one of four proteins that make up the influenza A virus replication complex, which is responsible for viral gene transcription and replication. To assess the antiviral potential of an anti-PB2 monoclonal antibody that inhibits RNA transcription of influenza A viruses, Mardin-Darby canine kidney (MDCK) cells were transformed with two transgenes that encode the light and heavy chains of the monoclonal antibody. The transformed cell lines expressing this monoclonal antibody displayed resistance to several subtypes of influenza A virus infection. In the transformed cell lines infected with influenza A virus, the level of viral RNA transcription was decreased and the effective nuclear transportation of the PB2 protein was also inhibited. These results demonstrate that the anti-PB2 intrabody is potentially able to interfere with the effective nuclear transportation of PB2 protein, resulting in the observed resistance to influenza A virus infection in vitro.

本文言語英語
ページ(範囲)487-493
ページ数7
ジャーナルVeterinary Journal
198
2
DOI
出版ステータス出版済み - 11 2013

All Science Journal Classification (ASJC) codes

  • 動物科学および動物学
  • 獣医学(全般)

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