TY - JOUR
T1 - Response regulator YycF essential for bacterial growth
T2 - X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif
AU - Okajima, Toshihide
AU - Doi, Akihiro
AU - Okada, Ario
AU - Gotoh, Yasuhiro
AU - Tanizawa, Katsuyuki
AU - Utsumi, Ryutaro
N1 - Funding Information:
This work has been supported by the Research and Development Program for New Bio-industry Initiatives (2006–2010) of Bio-oriented Technology Research Advancement Institution (BRAIN), Japan. The radiation experiment was done at the BL44XU in SPring-8 with the approval of the Japan Synchrotron Radiation Research Institute (JASRI).
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/10/15
Y1 - 2008/10/15
N2 - A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.
AB - A response regulator YycF and its cognate sensor kinase YycG constitute the two-component signal transduction system essential for growth of Gram-positive bacteria with a low GC content. We have determined the X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding. The structure, containing a winged helix-turn-helix motif, was found to be very similar to that of the response regulator PhoB from Escherichia coli. Specific binding of YycF to the PhoB-regulated alkaline phosphatase promoter was also demonstrated.
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U2 - 10.1016/j.febslet.2008.09.007
DO - 10.1016/j.febslet.2008.09.007
M3 - Article
C2 - 18789936
AN - SCOPUS:53049099975
VL - 582
SP - 3434
EP - 3438
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 23-24
ER -