Restoration of T cell development in RAG-2-deficient mice by functional TCR transgenes

Yoichi Shinkai, Shigeo Koyasu, Kei Ichi Nakayama, Kenneth M. Murphy, Dennis Y. Loh, Ellis L. Reinherz, Frederick W. Alt

研究成果: ジャーナルへの寄稿記事

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Introduction of TCRα transgene, TCRβ transgene, or both into RAG-2-/- mice differentially rescues T cell development. RAG-2 -/- mice have small numbers of TCR-CD4-CD8 - (double negative, DN) thymocytes that express CD3γδ ∈ and ζ proteins intracellularly. Introduction of a TCRβ transgene, but not a TCRα transgene, into the RAG-2-/- back-ground restored normal numbers of thymocytes. These cells were CD4 +CD8+ (double positive, DP) and expressed small amounts of surface TCRβ chain dimers in association with CD3γδ∈ but not ζ. RAG-2-/- mice that expressed α and β TCR transgenes developed both DP and single positive thymocytes. Thus, the TCRβ subunit, possibly in association with a novel CD3 complex, participates in the DN to the DP transition.

元の言語英語
ページ(範囲)822-825
ページ数4
ジャーナルScience
259
発行部数5096
DOI
出版物ステータス出版済み - 1 1 1993

All Science Journal Classification (ASJC) codes

  • General

フィンガープリント Restoration of T cell development in RAG-2-deficient mice by functional TCR transgenes' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Shinkai, Y., Koyasu, S., Nakayama, K. I., Murphy, K. M., Loh, D. Y., Reinherz, E. L., & Alt, F. W. (1993). Restoration of T cell development in RAG-2-deficient mice by functional TCR transgenes. Science, 259(5096), 822-825. https://doi.org/10.1126/science.8430336