Restricted diversification of T-cells in chronic active Epstein-Barr virus infection: Potential inclination to T-lymphoproliferative disease

Shouichi Ohga, Nobuhiro Kimura, Hidetoshi Takada, Mituyuki Nagano, Kohichi Ohshima, Akihiko Nomura, Kenji Muraoka, Hiromichi Take, Shunji Yamamori, Toshiro Hara

研究成果: ジャーナルへの寄稿学術誌査読

6 被引用数 (Scopus)

抄録

To assess the abnormal T-cell expansion in chronic active Epstein-Barr virus infection (CAEBV), T-cell antigen receptor (TCR) repertoire was analyzed in four patients with the disease. All fulfilled the diagnostic criteria of CAEBV, presenting with fever, hepatosplenomegaly, cytopenia, abnormal high titers of anti EBV-antibodies, and positive EBV genome of unknown cause. Southern blotting probed with EBV-terminal repeats and TCR Cβ gene indicated clonal expansion of the infected cells in 3 and 2 patients, respectively. The number of CD4+ HLA-DR+ cells appreciably increased in patients 1 (59%) and 2 (24%), who had a coronary aneurysm and central nervous system involvement, respectively. TCR gene expression examined by the inverse polymerase chain reaction methods revealed that Vβ gene usages were preferential in all patients (Vβ7 and Vβ12: patient 1, Vβ4: patient 2, Vβ13: patients 3 and 4), compared with those in healthy controls. Vα18 gene expression was remarkably high in patients 1 and 2. Moreover, Jβ gene expression was skewing in the reigning Vβ clones in all patients. Vβ4- Jβ1.5 and Vβ13-Jβ1.5 genes were clonally expressed in patients 2 and 4, respectively. These results suggest that CAEBV is associated with the restricted diversity of T-cells, which may stem from the sustained expansion of oligoclonal T-cells possibly driven by conventional viral antigens, but not, superantigens. Although the study is limited by the small number of patients, the unbalanced T-cell repertoire might contribute to the evolution of T-lymphoproliferative disease, otherwise, imply the innate defective immunity to EBV in CAEBV patients.

本文言語英語
ページ(範囲)26-33
ページ数8
ジャーナルAmerican Journal of Hematology
61
1
DOI
出版ステータス出版済み - 1999

!!!All Science Journal Classification (ASJC) codes

  • 血液学

フィンガープリント

「Restricted diversification of T-cells in chronic active Epstein-Barr virus infection: Potential inclination to T-lymphoproliferative disease」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル