Retroviral Gene Transduction into Chicken Embryo Gonads through Blood Circulation

Yoshinori Kawabe, Tsutomu Naka, Hiroyuki Komatsu, Ken Ichi Nishijima, Shinji Iijima, Masamichi Kamihira

研究成果: ジャーナルへの寄稿学術誌査読

5 被引用数 (Scopus)

抄録

A retroviral vector was injected into the heart of developing chicken embryos to determine the best timing for effective gene transduction into the gonads. Transduction efficiency and transgene expression were both highest when the viral solution was injected into embryos at stages 14-15 (Hamburger-Hamilton stages), in which primordial germ cells (PGCs) can be found in the blood circulation. This study shows that PGCs in the blood are important targets for gene transfer into the gonads using retroviral vectors.

本文言語英語
ページ(範囲)598-601
ページ数4
ジャーナルJournal of Bioscience and Bioengineering
106
6
DOI
出版ステータス出版済み - 12月 2008

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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