Revealing Spleen Ad4BP/SF1 Knockout Mouse by BAC-Ad4BP-tTAZ Transgene

Fatchiyah, M. Zubair, Ken-Ichirou Morohashi

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

1 被引用数 (Scopus)

抄録

The purpose of this study is addressed the function of Ad4BP/SF1 in developing spleen of Ad4BP/SF-1 KO mouse. I have modified the BAC-Ad4BP endogenous by homologous recombinant with modification cassette-containing tetracycline transactivator system and LacZ reporter genes to allow the expression of Ad4BP/SF1 in developing tissues and organ differentiation, regulate of Ad4BP/SF1 mechanism, and reveal the defect due to Ad4BP/SF1 deficiency. Recently studies, by immunihistochemistry of adult tissues showed that revealed coincident expressing between Ad4BP/SF-1 and lacZ, indicating the tTA system seemed to reproduces the endogenous expression of Ad4BP/SF-1, and thus likely to lacZ, Ad4BP/SF-1 in the BAC transgene was expected to be expressed in the corresponding tissues under the control of the bidirectional promoter. Interestingly, the BAC-Ad4BP-tTA transgenic mice were succeeded to up regulate the expression of Ad4BP/SF-1 at the protein level than wild type. And the fetal spleen of Tg(+);Ad4(+/+) was larger than that of the wild type, and expectedly the size of the spleen of Tg(+);Ad4(-/-) apparently recovered.

本文言語英語
ホスト出版物のタイトル13th International Conference on Biomedical Engineering - ICBME 2008
ページ1920-1923
ページ数4
DOI
出版ステータス出版済み - 12月 1 2009
イベント13th International Conference on Biomedical Engineering, ICBME 2008 - , シンガポール
継続期間: 12月 3 200812月 6 2008

出版物シリーズ

名前IFMBE Proceedings
23
ISSN(印刷版)1680-0737

その他

その他13th International Conference on Biomedical Engineering, ICBME 2008
国/地域シンガポール
Period12/3/0812/6/08

!!!All Science Journal Classification (ASJC) codes

  • 生体医工学
  • バイオエンジニアリング

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