TY - JOUR
T1 - Ribosomes in a stacked array
T2 - Elucidation of the step in translation elongation at which they are stalled during S-adenosyl-l-methionine-induced translation arrest of CGS1 mRNA
AU - Yamashita, Yui
AU - Kadokura, Yoshitomo
AU - Sotta, Naoyuki
AU - Fujiwara, Toru
AU - Takigawa, Ichigaku
AU - Satake, Akiko
AU - Onouchi, Hitoshi
AU - Naito, Satoshi
PY - 2014
Y1 - 2014
N2 - Background: S-Adenosyl-L-methionine arrests ribosomes on CGS1 mRNA at the pre-translocation step, and ribosomes stack behind it. Results: Puromycin reactivity of stacked ribosomes was intermediate between ribosomes at the pre- and post-translocation steps. Conclusion: Ribosomes are stacked at nine-codon intervals, and the majority are stalled at an early step of translocation. Significance: This study is the first report of ribosomal states in a stacked array.
AB - Background: S-Adenosyl-L-methionine arrests ribosomes on CGS1 mRNA at the pre-translocation step, and ribosomes stack behind it. Results: Puromycin reactivity of stacked ribosomes was intermediate between ribosomes at the pre- and post-translocation steps. Conclusion: Ribosomes are stacked at nine-codon intervals, and the majority are stalled at an early step of translocation. Significance: This study is the first report of ribosomal states in a stacked array.
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U2 - 10.1074/jbc.M113.526616
DO - 10.1074/jbc.M113.526616
M3 - Article
C2 - 24652291
AN - SCOPUS:84899728734
VL - 289
SP - 12693
EP - 12704
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 18
ER -