Rinse and evaporation coating of poly(methyl methacrylate) microchip for separation of sodium dodecyl sulfate-protein complex

Hiroki Okada, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)

抄録

We developed a novel channel wall coating on a poly(methyl methacrylate) (PMMA) microchip using methylcellulose (MC) as a coating reagent to suppress electroosmotic flow (EOF) following the strong analytes adsorption via hydrophobic interaction with channel walls of PMMA. Our coating was obtained by first rinsing channel walls with MC-containing aqueous solution followed by evaporation. The coating made the hydrophilic channel wall lowering EOF by two orders of magnitude (1.2 × 10-5 cm2 V-1 s-1) as well as reducing the hydrophobic adsorption. On the coated channel walls, we successfully separated sodium dodecyl sulfate-protein complexes with high reproducibility and efficiency using dextran as a lower viscosity protein separation medium.

本文言語英語
ページ(範囲)289-293
ページ数5
ジャーナルJournal of Chromatography A
1192
2
DOI
出版ステータス出版済み - 5月 30 2008
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 有機化学

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