RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells

Li Tian, Hong Li Chou, Laining Zhang, Seon Kap Hwang, Shawn R. Starkenburg, Kelly A. Doroshenk, Toshihiro Kumamaru, Thomas W. Okita

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

In developing rice (Oryza sativa) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported and anchored to distinct subdomains of the cortical endoplasmic reticulum. RNA binding protein RBP-P binds to both glutelin and prolamine mRNAs, suggesting a role in some aspect of their RNA metabolism. Here, we show that rice lines expressing mutant RBP-P mislocalize both glutelin and prolamine mRNAs. Different mutant RBP-P proteins exhibited varying degrees of reduced RNA binding and/or protein-protein interaction properties, which may account for the mislocalization of storage protein RNAs. In addition, partial loss of RBP-P function conferred a broad phenotypic variation ranging from dwarfism, chlorophyll deficiency, and sterility to late flowering and low spikelet fertility. Transcriptome analysis highlighted the essential role of RBP-P in regulating storage protein genes and several essential biological processes during grain development. Overall, our data demonstrate the significant roles of RBP-P in glutelin and prolamine mRNA localization and in the regulation of genes important for plant growth and development through its RNA binding activity and cooperative regulation with interacting proteins.

元の言語英語
ページ(範囲)2529-2552
ページ数24
ジャーナルThe Plant cell
30
発行部数10
DOI
出版物ステータス出版済み - 10 1 2018

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Phenylpropanolamine
Endosperm
glutelins
RNA-binding proteins
RNA-Binding Proteins
Glutens
endosperm
storage proteins
RNA
rice
Messenger RNA
Proteins
cells
mutants
dwarfing
protein-protein interactions
seed development
transcriptomics
phenotypic variation
endoplasmic reticulum

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

これを引用

Tian, L., Chou, H. L., Zhang, L., Hwang, S. K., Starkenburg, S. R., Doroshenk, K. A., ... Okita, T. W. (2018). RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells. The Plant cell, 30(10), 2529-2552. https://doi.org/10.1105/tpc.18.00321

RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells. / Tian, Li; Chou, Hong Li; Zhang, Laining; Hwang, Seon Kap; Starkenburg, Shawn R.; Doroshenk, Kelly A.; Kumamaru, Toshihiro; Okita, Thomas W.

:: The Plant cell, 巻 30, 番号 10, 01.10.2018, p. 2529-2552.

研究成果: ジャーナルへの寄稿記事

Tian, L, Chou, HL, Zhang, L, Hwang, SK, Starkenburg, SR, Doroshenk, KA, Kumamaru, T & Okita, TW 2018, 'RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells', The Plant cell, 巻. 30, 番号 10, pp. 2529-2552. https://doi.org/10.1105/tpc.18.00321
Tian L, Chou HL, Zhang L, Hwang SK, Starkenburg SR, Doroshenk KA その他. RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells. The Plant cell. 2018 10 1;30(10):2529-2552. https://doi.org/10.1105/tpc.18.00321
Tian, Li ; Chou, Hong Li ; Zhang, Laining ; Hwang, Seon Kap ; Starkenburg, Shawn R. ; Doroshenk, Kelly A. ; Kumamaru, Toshihiro ; Okita, Thomas W. / RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells. :: The Plant cell. 2018 ; 巻 30, 番号 10. pp. 2529-2552.
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abstract = "In developing rice (Oryza sativa) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported and anchored to distinct subdomains of the cortical endoplasmic reticulum. RNA binding protein RBP-P binds to both glutelin and prolamine mRNAs, suggesting a role in some aspect of their RNA metabolism. Here, we show that rice lines expressing mutant RBP-P mislocalize both glutelin and prolamine mRNAs. Different mutant RBP-P proteins exhibited varying degrees of reduced RNA binding and/or protein-protein interaction properties, which may account for the mislocalization of storage protein RNAs. In addition, partial loss of RBP-P function conferred a broad phenotypic variation ranging from dwarfism, chlorophyll deficiency, and sterility to late flowering and low spikelet fertility. Transcriptome analysis highlighted the essential role of RBP-P in regulating storage protein genes and several essential biological processes during grain development. Overall, our data demonstrate the significant roles of RBP-P in glutelin and prolamine mRNA localization and in the regulation of genes important for plant growth and development through its RNA binding activity and cooperative regulation with interacting proteins.",
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AU - Starkenburg, Shawn R.

AU - Doroshenk, Kelly A.

AU - Kumamaru, Toshihiro

AU - Okita, Thomas W.

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