RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

Jae Man Lee; Yoshito Kojin; Tsuneyuki Tatsuke; Hiroaki Mon; Yoshitaka Miyagawa; Takahiro Kusakabe

RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

Jae Man Lee, Yoshito Kojin, Tsuneyuki Tatsuke, Hiroaki Mon, Yoshitaka Miyagawa, Takahiro Kusakabe

農業生物科学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

1 被引用数 (Scopus)

抄録

RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.

本文言語	英語
ページ（範囲）	441-445
ページ数	5
ジャーナル	Journal of the Faculty of Agriculture, Kyushu University
巻	57
号	2
出版ステータス	出版済み - 9月 2012

!!!All Science Journal Classification (ASJC) codes

農業および作物学
バイオテクノロジー

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引用スタイル

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title = "RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells",

abstract = "RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway{\textregistered}-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.",

author = "Lee, {Jae Man} and Yoshito Kojin and Tsuneyuki Tatsuke and Hiroaki Mon and Yoshitaka Miyagawa and Takahiro Kusakabe",

year = "2012",

month = sep,

language = "English",

volume = "57",

pages = "441--445",

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T1 - RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

AU - Lee, Jae Man

AU - Kojin, Yoshito

AU - Tatsuke, Tsuneyuki

AU - Mon, Hiroaki

AU - Miyagawa, Yoshitaka

AU - Kusakabe, Takahiro

PY - 2012/9

Y1 - 2012/9

N2 - RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.

AB - RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.

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M3 - Article

AN - SCOPUS:84866674786

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JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

IS - 2

ER -

RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

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!!!All Science Journal Classification (ASJC) codes

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