RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells

Jae Man Lee, Yoshito Kojin, Tsuneyuki Tatsuke, Hiroaki Mon, Yoshitaka Miyagawa, Takahiro Kusakabe

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

RNA interference (RNAi) triggered by long double-stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high-throughput analyses of gene function. In this report, we have constructed Gateway®-based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70-80% in cultured B. mori cells.

本文言語英語
ページ(範囲)441-445
ページ数5
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
57
2
出版ステータス出版済み - 9 2012

All Science Journal Classification (ASJC) codes

  • 農業および作物学
  • バイオテクノロジー

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