RNA polymerase II bypasses 8-oxoguanine in the presence of transcription elongation factor TFIIS

Isao Kuraoka, Kyoko Suzuki, Shinsuke Ito, Mika Hayashida, Joan Seah Mei Kwei, Takahisa Ikegami, Hiroshi Handa, Yusaku Nakabeppu, Kiyoji Tanaka

研究成果: Contribution to journalArticle査読

59 被引用数 (Scopus)

抄録

The blockage of transcription elongation by RNA polymerase II (RNAPII) at DNA lesions on the transcribed strand is a serious challenge to accurate transcription. Transcription-coupled DNA repair (TCR), which is assumed to be initiated by the blockage of transcription, rapidly removes lesions on the transcribed strand of expressed genes and allows the resumption of transcription. Although helix-distorting bulky damage such as a cyclobutane pyrimidine dimer is known to block transcription elongation and to be repaired by TCR, it is not clear whether oxidative DNA lesions are repaired by TCR. First, we examined whether transcription elongation by RNAPII is stalled at sites of 2-hydroxyadenine (2-OH-A), 8-oxoadenine (8-oxoA), 8-oxoguanine (8-oxoG), or thymine glycol (Tg) on the transcribed strand. Our results indicate that RNAPII incorporated nucleotides opposite the lesions and then stalled. In addition, we found that transcription elongation factor TFIIS (SII) enabled RNAPII to bypass 8-oxoG but not the other types of damage, while transcription initiation and elongation factor TFIIF did not bypass 8-oxoG. These results suggest that SII is important for preventing cellular death due to oxidative DNA damage, assisting RNAPII to bypass 8-oxoG.

本文言語英語
ページ(範囲)841-851
ページ数11
ジャーナルDNA Repair
6
6
DOI
出版ステータス出版済み - 6 1 2007

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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