RNA-primed PCR

Hiroki Shibata, Tomoko Tahira, Kenshi Hayashi

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of the target nucleotide sequence.

元の言語英語
ページ(範囲)400-403
ページ数4
ジャーナルGenome Research
5
発行部数4
出版物ステータス出版済み - 11 1 1995

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RNA
Polymerase Chain Reaction
DNA Primers
RNA-Directed DNA Polymerase
DNA-Directed DNA Polymerase
RNA primers
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

これを引用

Shibata, H., Tahira, T., & Hayashi, K. (1995). RNA-primed PCR. Genome Research, 5(4), 400-403.

RNA-primed PCR. / Shibata, Hiroki; Tahira, Tomoko; Hayashi, Kenshi.

:: Genome Research, 巻 5, 番号 4, 01.11.1995, p. 400-403.

研究成果: ジャーナルへの寄稿記事

Shibata, H, Tahira, T & Hayashi, K 1995, 'RNA-primed PCR', Genome Research, 巻. 5, 番号 4, pp. 400-403.
Shibata H, Tahira T, Hayashi K. RNA-primed PCR. Genome Research. 1995 11 1;5(4):400-403.
Shibata, Hiroki ; Tahira, Tomoko ; Hayashi, Kenshi. / RNA-primed PCR. :: Genome Research. 1995 ; 巻 5, 番号 4. pp. 400-403.
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