RNA targeting to a specific ER sub-domain is required for efficient transport and packaging of α-globulins to the protein storage vacuole in developing rice endosperm

Haruhiko Washida, Aya Sugino, Kelly A. Doroshenk, Mio Satoh-Cruz, Ai Nagamine, Tomoyuki Katsube-Tanaka, Masahiro Ogawa, Toshihiro Kumamaru, Hikaru Satoh, Thomas W. Okita

研究成果: ジャーナルへの寄稿記事

24 引用 (Scopus)

抄録

Studies focusing on the targeting of RNAs that encode rice storage proteins, prolamines and glutelins to specific sub-domains of the endoplasmic reticulum (ER), as well as mis-localization studies of other storage protein RNAs, indicate a close relationship between the ER site of RNA translation and the final site of protein deposition in the endomembrane system in developing rice endosperm. In addition to prolamine and glutelin, rice accumulates smaller amounts of α-globulins, which are deposited together with glutelin in the protein storage vacuole (PSV). In situ RT-PCR analysis revealed that α-globulin RNAs are not distributed to the cisternal ER as expected for a PSV-localized protein, but instead are targeted to the protein body-ER (PB-ER) by a regulated process requiring cis-sorting sequences. Sequence alignments with putative maize Î-zein cis-localization elements identified several candidate regulatory sequences that may be responsible for PB-ER targeting. Immunocytochemical analysis confirmed the presence of α-globulin on the periphery of the prolamine protein bodies and packaging in Golgi-associated dense vesicles, as well as deposition and storage within peripheral regions of the PSV. Mis-targeting of α-globulin RNAs to the cisternal ER dramatically alters the spatial arrangement of α-globulin and glutelin within the PSV, with the accompanying presence of numerous small α-globulin particles in the cytoplasm. These results indicate that α-globulin RNA targeting to the PB-ER sub-domain is essential for efficient transport of α-globulins to the PSV and its spatial arrangement in the PSV. Such RNA localization prevents potential deleterious protein-protein interactions, in addition to performing a role in protein targeting.

元の言語英語
ページ(範囲)471-479
ページ数9
ジャーナルPlant Journal
70
発行部数3
DOI
出版物ステータス出版済み - 5 1 2012

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Endosperm
Globulins
Product Packaging
storage proteins
Vacuoles
globulins
Endoplasmic Reticulum
endoplasmic reticulum
packaging
endosperm
vacuoles
RNA
rice
glutelins
protein bodies
Proteins
Glutens
Phenylpropanolamine
endomembrane system
rice protein

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

これを引用

RNA targeting to a specific ER sub-domain is required for efficient transport and packaging of α-globulins to the protein storage vacuole in developing rice endosperm. / Washida, Haruhiko; Sugino, Aya; Doroshenk, Kelly A.; Satoh-Cruz, Mio; Nagamine, Ai; Katsube-Tanaka, Tomoyuki; Ogawa, Masahiro; Kumamaru, Toshihiro; Satoh, Hikaru; Okita, Thomas W.

:: Plant Journal, 巻 70, 番号 3, 01.05.2012, p. 471-479.

研究成果: ジャーナルへの寄稿記事

Washida, Haruhiko ; Sugino, Aya ; Doroshenk, Kelly A. ; Satoh-Cruz, Mio ; Nagamine, Ai ; Katsube-Tanaka, Tomoyuki ; Ogawa, Masahiro ; Kumamaru, Toshihiro ; Satoh, Hikaru ; Okita, Thomas W. / RNA targeting to a specific ER sub-domain is required for efficient transport and packaging of α-globulins to the protein storage vacuole in developing rice endosperm. :: Plant Journal. 2012 ; 巻 70, 番号 3. pp. 471-479.
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abstract = "Studies focusing on the targeting of RNAs that encode rice storage proteins, prolamines and glutelins to specific sub-domains of the endoplasmic reticulum (ER), as well as mis-localization studies of other storage protein RNAs, indicate a close relationship between the ER site of RNA translation and the final site of protein deposition in the endomembrane system in developing rice endosperm. In addition to prolamine and glutelin, rice accumulates smaller amounts of α-globulins, which are deposited together with glutelin in the protein storage vacuole (PSV). In situ RT-PCR analysis revealed that α-globulin RNAs are not distributed to the cisternal ER as expected for a PSV-localized protein, but instead are targeted to the protein body-ER (PB-ER) by a regulated process requiring cis-sorting sequences. Sequence alignments with putative maize {\^I}-zein cis-localization elements identified several candidate regulatory sequences that may be responsible for PB-ER targeting. Immunocytochemical analysis confirmed the presence of α-globulin on the periphery of the prolamine protein bodies and packaging in Golgi-associated dense vesicles, as well as deposition and storage within peripheral regions of the PSV. Mis-targeting of α-globulin RNAs to the cisternal ER dramatically alters the spatial arrangement of α-globulin and glutelin within the PSV, with the accompanying presence of numerous small α-globulin particles in the cytoplasm. These results indicate that α-globulin RNA targeting to the PB-ER sub-domain is essential for efficient transport of α-globulins to the PSV and its spatial arrangement in the PSV. Such RNA localization prevents potential deleterious protein-protein interactions, in addition to performing a role in protein targeting.",
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AU - Washida, Haruhiko

AU - Sugino, Aya

AU - Doroshenk, Kelly A.

AU - Satoh-Cruz, Mio

AU - Nagamine, Ai

AU - Katsube-Tanaka, Tomoyuki

AU - Ogawa, Masahiro

AU - Kumamaru, Toshihiro

AU - Satoh, Hikaru

AU - Okita, Thomas W.

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Y1 - 2012/5/1

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AB - Studies focusing on the targeting of RNAs that encode rice storage proteins, prolamines and glutelins to specific sub-domains of the endoplasmic reticulum (ER), as well as mis-localization studies of other storage protein RNAs, indicate a close relationship between the ER site of RNA translation and the final site of protein deposition in the endomembrane system in developing rice endosperm. In addition to prolamine and glutelin, rice accumulates smaller amounts of α-globulins, which are deposited together with glutelin in the protein storage vacuole (PSV). In situ RT-PCR analysis revealed that α-globulin RNAs are not distributed to the cisternal ER as expected for a PSV-localized protein, but instead are targeted to the protein body-ER (PB-ER) by a regulated process requiring cis-sorting sequences. Sequence alignments with putative maize Î-zein cis-localization elements identified several candidate regulatory sequences that may be responsible for PB-ER targeting. Immunocytochemical analysis confirmed the presence of α-globulin on the periphery of the prolamine protein bodies and packaging in Golgi-associated dense vesicles, as well as deposition and storage within peripheral regions of the PSV. Mis-targeting of α-globulin RNAs to the cisternal ER dramatically alters the spatial arrangement of α-globulin and glutelin within the PSV, with the accompanying presence of numerous small α-globulin particles in the cytoplasm. These results indicate that α-globulin RNA targeting to the PB-ER sub-domain is essential for efficient transport of α-globulins to the PSV and its spatial arrangement in the PSV. Such RNA localization prevents potential deleterious protein-protein interactions, in addition to performing a role in protein targeting.

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