抄録
In aerobically growing cells, in which reactive oxygen species are produced, the guanine base of RNA is oxidized to 8-oxo-7,8-dihydroguanine, which induces alterations in gene expression. Here we show that the human Auf1 protein, also called HNRNPD, binds specifically to RNA containing this oxidized base and may be involved in cellular processes associated with managing the problems caused by RNA oxidation. Auf1-deficient cells were constructed from human HeLa and Nalm-6 lines using two different targeting procedures. Both types of Auf1-deficient cells are viable, but exhibit growth retardation. The stability of messenger RNA for four different housekeeping genes was determined in Auf1-deficient and -proficient cells, treated with or without hydrogen peroxide. The level of oxidized messenger RNA was considerably higher in Auf1-deficient cells than in Auf1-proficient cells. Auf1 may play a role in the elimination of oxidized RNA, which is required for the maintenance of proper gene expression under conditions of oxidative stress.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 109-116 |
| ページ数 | 8 |
| ジャーナル | Free Radical Biology and Medicine |
| 巻 | 79 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2015 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Physiology (medical)
これを引用
Role of Auf1 in elimination of oxidatively damaged messenger RNA in human cells. / Ishii, Takashi; Hayakawa, Hiroshi; Sekiguchi, Takeshi; Adachi, Noritaka; Sekiguchi, Mutsuo.
：: Free Radical Biology and Medicine, 巻 79, 01.01.2015, p. 109-116.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Role of Auf1 in elimination of oxidatively damaged messenger RNA in human cells
AU - Ishii, Takashi
AU - Hayakawa, Hiroshi
AU - Sekiguchi, Takeshi
AU - Adachi, Noritaka
AU - Sekiguchi, Mutsuo
PY - 2015/1/1
Y1 - 2015/1/1
N2 - In aerobically growing cells, in which reactive oxygen species are produced, the guanine base of RNA is oxidized to 8-oxo-7,8-dihydroguanine, which induces alterations in gene expression. Here we show that the human Auf1 protein, also called HNRNPD, binds specifically to RNA containing this oxidized base and may be involved in cellular processes associated with managing the problems caused by RNA oxidation. Auf1-deficient cells were constructed from human HeLa and Nalm-6 lines using two different targeting procedures. Both types of Auf1-deficient cells are viable, but exhibit growth retardation. The stability of messenger RNA for four different housekeeping genes was determined in Auf1-deficient and -proficient cells, treated with or without hydrogen peroxide. The level of oxidized messenger RNA was considerably higher in Auf1-deficient cells than in Auf1-proficient cells. Auf1 may play a role in the elimination of oxidized RNA, which is required for the maintenance of proper gene expression under conditions of oxidative stress.
AB - In aerobically growing cells, in which reactive oxygen species are produced, the guanine base of RNA is oxidized to 8-oxo-7,8-dihydroguanine, which induces alterations in gene expression. Here we show that the human Auf1 protein, also called HNRNPD, binds specifically to RNA containing this oxidized base and may be involved in cellular processes associated with managing the problems caused by RNA oxidation. Auf1-deficient cells were constructed from human HeLa and Nalm-6 lines using two different targeting procedures. Both types of Auf1-deficient cells are viable, but exhibit growth retardation. The stability of messenger RNA for four different housekeeping genes was determined in Auf1-deficient and -proficient cells, treated with or without hydrogen peroxide. The level of oxidized messenger RNA was considerably higher in Auf1-deficient cells than in Auf1-proficient cells. Auf1 may play a role in the elimination of oxidized RNA, which is required for the maintenance of proper gene expression under conditions of oxidative stress.
UR - http://www.scopus.com/inward/record.url?scp=84919595088&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84919595088&partnerID=8YFLogxK
U2 - 10.1016/j.freeradbiomed.2014.11.018
DO - 10.1016/j.freeradbiomed.2014.11.018
M3 - Article
VL - 79
SP - 109
EP - 116
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
SN - 0891-5849
ER -