Role of IL-10 in the crossregulation of prostaglandins and cytokines in monocytes

Y. Niho, H. Niiro, Y. Tanaka, H. Nakashima, T. Otsuka

研究成果: ジャーナルへの寄稿記事

40 引用 (Scopus)

抄録

In the present study we have focused mainly on the role of IL (interleukin)-10 in the crossregulation of prostaglandins and cytokines in human monocytes. We first determined the effects of tumor necrosis factor-α (TNF-α) and IL-10 on monocyte prostaglandin E2 (PGE2) production. Unstimulated monocytes constitutively produced a small but significant amount of PGE2 in the culture supernatants. Both TNF-α and lipopolysaccharide (LPS) caused a remarkable increase in monocyte PGE2 production. On the other hand, IL-10 alone was without effect on constitutive PGE2 production but drastically inhibited LPS-induced PGE2 production in monocytes. Moreover, this inhibitory effect of IL-10 was not simply attributable to its inhibition of TNF-α production in LPS-stimulated monocytes. Next, we determined the effect of PGE2 on TNF-α mRNA expression in monocytes. Treatment of monocytes with or without PGE2 showed no detectable TNF-α mRNA. Activation of monocytes by LPS resulted in a remarkable accumulation of TNF-α mRNA and PGE2 efficiently inhibited this expression. Finally, we determined the effect of PGE2 on IL-10 mRNA expression in monocytes. Similar to TNF-α mRNA, unstimulated monocytes showed no detectable IL-10 mRNA. Interestingly, PGE2 alone drastically induced IL-10 mRNA. Besides, activation of monocytes by LPS resulted in a remarkable accumulation of IL-10 mRNA, and PGE2 further enhanced this expression. These results indicate that TNF-α and PGE2 are key molecules for the induction of IL-10 in monocytes, and that IL-10, in turn, plays a crucial role in terminating the inflammatory cascade via downregulation of production of proinflammatory molecules including TNF-α and PGE2.

元の言語英語
ページ(範囲)165-170
ページ数6
ジャーナルActa Haematologica
99
発行部数3
DOI
出版物ステータス出版済み - 4 18 1998

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Dinoprostone
Interleukin-10
Prostaglandins
Monocytes
Cytokines
Tumor Necrosis Factor-alpha
Messenger RNA
Lipopolysaccharides
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Hematology

これを引用

Role of IL-10 in the crossregulation of prostaglandins and cytokines in monocytes. / Niho, Y.; Niiro, H.; Tanaka, Y.; Nakashima, H.; Otsuka, T.

:: Acta Haematologica, 巻 99, 番号 3, 18.04.1998, p. 165-170.

研究成果: ジャーナルへの寄稿記事

Niho, Y. ; Niiro, H. ; Tanaka, Y. ; Nakashima, H. ; Otsuka, T. / Role of IL-10 in the crossregulation of prostaglandins and cytokines in monocytes. :: Acta Haematologica. 1998 ; 巻 99, 番号 3. pp. 165-170.
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